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Journal of Neuroscience, Vol 4, 2329-2345, Copyright © 1984 by Society for Neuroscience
Pathfinding by neuronal growth cones in grasshopper embryos. IV. The effects of ablating the A and P axons upon the behavior of the G growth cone
JA Raper, MJ Bastiani and CS Goodman
In the companion paper (Bastiani, M. J., J. A. Raper, and C. S. Goodman
(1984) J. Neurosci. 4: 2311-2328), we show that as the G growth cone
reaches its choice point and turns anteriorly on the A/P fascicle, its
filopodia demonstrate selective affinity for the A/P fascicle as compared
to the other approximately 25 longitudinal axon fascicles, and within the
A/P fascicle itself, G's filopodia selectively contact the P axons as
compared to the A axons. These results support the hypothesis that the A/P
fascicle, and, moreover, subsets of axons within it (Ps versus As), are
specifically labeled and that the G growth cone is determined to follow a
particular labeled pathway. We tested the "labeled pathways" hypothesis by
specifically ablating these axons and examining the subsequent behavior of
the G growth cone in embryos grown in culture. Ablation of the A and P
axons prevents G from growing more than a short distance anteriorly,
although the G growth cone is within grasp of many other longitudinal axon
fascicles. Ablation of only the P axons has a similar effect; the G growth
cone behaves normally if only the A axons are ablated. Transmission
electron micrograph semiserial section reconstructions of experimental
embryos further indicate that G's growth cone behaves abnormally when the A
and P axons, or only the P axons, are ablated. The G growth cone branches
extensively in the lateral and ventral neuropil without it or its filopodia
showing a high affinity for any other axon fascicle. These results indicate
that the G growth cone is able to distinguish the A/P fascicle from the
other longitudinal axon fascicles in the developing neuropil. Moreover, the
results suggest that within the A/P fascicle the G growth cone is able to
distinguish the P axons from the A axons.
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