 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 4, 2354-2368, Copyright © 1984 by Society for Neuroscience
Alterations in neural cell adhesion molecules during development of different regions of the nervous system
CM Chuong and GM Edelman
Several cell adhesion molecules involved in neuron-neuron and neuron- glia
interactions have been identified in our laboratory and have been shown to
undergo cell surface modulation. In the case of the neural cell adhesion
molecule (N-CAM), it has been found that during development the molecule is
converted from a microheterogeneous embryonic (E) form containing 30 gm of
sialic acid/100 gm of polypeptide to several distinct adult (A) forms
containing one third as much of this sugar. In vitro analyses indicate that
this change is accompanied by a 4-fold increase in the rate of N-CAM
homophilic binding. In the present study of the mouse and the chick,
alterations of N-CAMs occurring as a result of E----A conversion,
prevalence modulation, and changes in antigenic state during the
development of different neural regions were analyzed by the use of highly
specific polyclonal and monoclonal antibodies combined with anatomical
dissection and several new quantitative assays. We made the following
observations. The relative concentration of N-CAM changed during
development, with the highest concentration (2.8 times the adult level)
occurring around the perinatal period. Each brain region followed a similar
pattern of change but according to a different time schedule. While
conversion from the E to the A forms of N-CAM occurred mainly during the
first 3 postnatal weeks in mice, the relative conversion rates were
distinctly different in various neural tissues. The extreme examples are
dorsal root ganglia, which already displayed the A forms at birth, and the
diencephalon and tectal region, which still retained some E forms in the
adult. A cephalocaudal maturation gradient of E---- A conversion was
observed in the spinal cord and dorsal root ganglia. Differences in the
antigenic determinants of N-CAMs from different neural tissues were
detected by two independent monoclonal antibodies. Finally, in some adult
neural tissues, one of the three A forms was found to be dominant. These
results establish that during development there are definite quantitative
and qualitative differences among N- CAMs from various neural tissues. The
data are consistent with the hypothesis that alterations in the relative
amounts and forms of N-CAM play major roles in neural morphogenesis,
possibly by altering the rates of adhesion among neurons and their
processes.
This article has been cited by other articles:
|
|
|
|
 
J. A. Murphy, A. T. E. Hartwick, U. Rutishauser, and D. B. Clarke
Endogenous Polysialylated Neural Cell Adhesion Molecule Enhances the Survival of Retinal Ganglion Cells
Invest. Ophthalmol. Vis. Sci.,
February 1, 2009;
50(2):
861 - 869.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Prosser, U. Rutishauser, G. Ungers, L. Fedorkova, and J. D. Glass
Intrinsic Role of Polysialylated Neural Cell Adhesion Molecule in Photic Phase Resetting of the Mammalian Circadian Clock
J. Neurosci.,
January 15, 2003;
23(2):
652 - 658.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Marx, U. Rutishauser, and M. Bastmeyer
Dual function of polysialic acid during zebrafish central nervous system development
Development,
December 15, 2001;
128(24):
4949 - 4958.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Shin, J. S. Lin, P. W. Anderson, R. A. Insel, and M. H. Nahm
Monoclonal Antibodies Specific for Neisseria meningitidis Group B Polysaccharide and Their Peptide Mimotopes
Infect. Immun.,
May 1, 2001;
69(5):
3335 - 3342.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Esni, I.-B. Taljedal, A.-K. Perl, H. Cremer, G. Christofori, and H. Semb
Neural Cell Adhesion Molecule (N-CAM) Is Required for Cell Type Segregation and Normal Ultrastructure in Pancreatic Islets
J. Cell Biol.,
January 25, 1999;
144(2):
325 - 337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Daston, M. Bastmeyer, U. Rutishauser, and D. D. M. O'Leary
Spatially Restricted Increase in Polysialic Acid Enhances Corticospinal Axon Branching Related to Target Recognition and Innervation
J. Neurosci.,
September 1, 1996;
16(17):
5488 - 5497.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X Yin, M Watanabe, and U Rutishauser
Effect of polysialic acid on the behavior of retinal ganglion cell axons during growth into the optic tract and tectum
Development,
January 10, 1995;
121(10):
3439 - 3446.
[Abstract]
[PDF]
|
|
|
|
|
|
|
P Doherty, S. Skaper, S. Moore, A Leon, and F. Walsh
A developmentally regulated switch in neuronal responsiveness to NCAM and N-cadherin in the rat hippocampus
Development,
January 7, 1992;
115(3):
885 - 892.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J Dodd and T. Jessell
Axon guidance and the patterning of neuronal projections in vertebrates
Science,
November 4, 1988;
242(4879):
692 - 699.
[Abstract]
[PDF]
|
|
|
|
|
|
|
B. Cunningham, J. Hemperly, B. Murray, E. Prediger, R Brackenbury, and G. Edelman
Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing
Science,
May 15, 1987;
236(4803):
799 - 806.
[Abstract]
[PDF]
|
|
|
|
|
|
|
G.M. Edelman
Cell Adhesion Molecule Expression and the Regulation of Morphogenesis
Cold Spring Harb Symp Quant Biol,
January 1, 1985;
50(0):
877 - 889.
[Abstract]
[PDF]
|
|
|
|
|
