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Journal of Neuroscience, Vol 5, 98-109, Copyright © 1985 by Society for Neuroscience
Neurophysin in the hypothalamo-neurohypophysial system. II. Immunocytochemical studies of the ontogeny of oxytocinergic and vasopressinergic neurons
MH Whitnall, S Key, Y Ben-Barak, K Ozato and H Gainer
Two anti-neurophysin monoclonal antibodies (MABs), PS 36 and PS 41,
described in the preceding paper (Ben-Barak, Y, J.T. Russell, M.H.
Whitnall, K. Ozato, and H. Gainer (1985) J. Neurosci. 5:000-000), allowed
us to specifically stain for oxytocin-associated neurophysin (NP-OT) or
vasopressin-associated neurophysin (NP-AVP) in the hypothalamus of
developing rats. Staining with these MABs specific for NP-OT or NP-AVP
showed that both types of neurophysin appeared in cells in the developing
hypothalamus as early as embryonic day (E16) and continued to increase in
immunoreactivity throughout fetal life. The literature indicated that
oxytocin appears in the system between E20 and E22, much later than
vasopressin (E16 to E17), which we confirmed in immunocytochemical
experiments using affinity-purified antisera to these hormones. Since the
MABs recognize the specific prohormones as well as the specific mature
neurophysins (Ben-Barak, Y., J. T. Russell, M.H. Whitnall, K. Ozato, and H.
Gainer (1985) J. Neurosci. 5: 81-97), we conclude that there is a
developmental delay between the synthesis of the oxytocin prohormone
(pro-oxyphysin) and its processing to form oxytocin and NP-OT. The delay in
prohormone processing in the oxytocin cells was correlated with a delay in
immunocytochemically detectable neurites as compared to the vasopressin
cells. This reduced level of axonal and dendritic immunoreactivity was
still obvious in the oxytocin cells at 9 days after birth. In contrast, the
clustering of cells to form adult-like hypothalamic nuclei appeared to
follow similar time courses for the two types of cells. Adult-like
distributions of cells staining for NP-OT and NP-AVP were already apparent
in the supraoptic and paraventricular nuclei by E17.
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