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Journal of Neuroscience, Vol 5, 2726-2731, Copyright © 1985 by Society for Neuroscience
Characterization of a component in chick ciliary ganglia that cross- reacts with monoclonal antibodies to muscle and electric organ acetylcholine receptor
MA Smith, J Stollberg, JM Lindstrom and DK Berg
Chick ciliary ganglion neurons have previously been shown to contain a
component that shares an antigenic determinant with the "main immunogenic
region" of the alpha-subunit in nicotinic acetylcholine receptor from
skeletal muscle and electric organ. Ultrastructural studies of antibody
binding in the ganglion have shown that the cross- reacting antigen exposed
on the surface of the neurons is located predominantly in synaptic
membrane. Here we show that the neuronal antigen can be identified in
detergent extracts of ciliary and sympathetic ganglia, but not in extracts
of heart, liver, spinal cord, retina, or dorsal root ganglia. In the
ciliary ganglion the component is present as an integral membrane
constituent, and, when detergent solubilized, it sediments as a 10 S
species and binds to concanavalin A. The component is distinct from the
alpha-bungarotoxin-binding site on the neurons since toxin-binding sites
and antibody-binding sites can be precipitated separately in ganglion
extracts. The component reaches peak levels per ganglionic protein between
embryonic days 8 and 12. These are some of the properties expected for the
nicotinic acetylcholine receptor on ciliary ganglion neurons.
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