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Journal of Neuroscience, Vol 5, 3124-3134, Copyright © 1985 by Society for Neuroscience
The neuronal endomembrane system. II. The multiple forms of the Golgi apparatus cis element
JD Lindsey and MH Ellisman
The cis element and its associated tubules and vesicles are ideally
positioned to play a major role in the sorting of rough endoplasmic
reticulum components destined for processing in the Golgi apparatus. Its
position is also ideal for playing a major role in the assembly of the
saccules which constitute the Golgi apparatus. The present study was
undertaken to critically analyze the normal morphology of this Golgi
apparatus component. Seventy- to 2000-nm-thick sections of bullfrog spinal
ganglia, fixed by osmium impregnation as well as by conventional protocols,
were studied using standard and high voltage electron microscopy.
Impregnated cis elements were also reconstructed from 170-nm serial
sections. These studies found that adjacent neurons within the given
ganglion contain cis elements of widely different morphology. In larger
neurons, a different cis element organization was also found in different
regions of the same cells. Based on structural comparisons, all of the
different cis element forms observed could be systematically assembled into
a gradual continuum of morphological variation. This continuum was circular
in a manner analogous to chromosomal variations seen in highly mitotic
tissues. For the sake of discussion, five distinctly different form
categories were established. Some forms contained structures that are
described herein for the first time. Most notable among these is the cis
tubular network, an extensive system of parallel tubules that closely
apposed the regularly perforated cis saccule. Osmiophilic vesicles were
found to collect in tight clusters that closely apposed certain forms of
the cis element. These findings raise the possibility that the cis element
and its associated structures may undergo morphological transformations as
part of their normal function.
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