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Journal of Neuroscience, Vol 5, 3310-3328, Copyright © 1985 by Society for Neuroscience
Identification of major cell classes in the developing mammalian nervous system
S Hockfield and RD McKay
A major difficulty in studying early developmental processes and testing
hypotheses of possible cellular mechanisms of development has been the
inability to reproducibly identify specific cell types. We have generated
monoclonal antibodies that distinguish among major cell types present
during mammalian neurogenesis. These antibodies have been used to analyze
the development of cellular organization in the early nervous system.
Monoclonal antibody Rat-401 identifies a transient radial glial cell in the
embryonic rat central nervous system (CNS) that is temporally and spatially
suited to guide neuronal migration. Rat-401 also identifies a peripheral
non-neuronal cell that may establish axon routes from the CNS to the
periphery. Monoclonal antibody Rat-202 recognizes an antigen present in
early axons, their growth cones, and filopodia, and has allowed us to
follow early axons and observe the structures they contact. Two other
antibodies that recognize axons demonstrate antigenically distinct phases
in axon development. In addition, we report a marker for another cell class
present in the developing nervous system, the endothelial cells that give
rise to the CNS vasculature.
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