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Journal of Neuroscience, Vol 5, 438-449, Copyright © 1985 by Society for Neuroscience

ARTICLE

A panel of monoclonal antibodies to the rat olfactory epithelium

JL Hempstead and JI Morgan

BALB/c mice were immunized with dissected olfactory mucosa from young adult Sprague-Dawley-derived, CD, rats and antibody-secreting hybridomas were produced. Supernatants from hybridoma cultures were screened by immunocytochemical methods for their ability to react with specific cell populations in frozen sections of rat olfactory epithelium. Approximately 60 clones were identified that showed various degrees of specific staining. These have been classified on the basis of their particular staining specificities. One group of monoclonal antibodies, designated LUM, reacts with the luminal surface of the epithelium. Closer examination reveals these antibodies to react variously with the apical brush border of sustentacular cells, respiratory cilia, and the luminal membrane of respiratory cells. Another group of monoclonal antibodies reacts primarily with sustentacular cells, as is indicated by the SUS prefix. Some antibodies in this group also react with the membranes of respiratory cells and the cells comprising the acini of Bowman's glands. A larger group of antibodies reacts with olfactory neurons and/or their axons, as denoted by the NEU prefix. This group can be further subdivided by the criteria of whether both the olfactory nerve and vomeronasal nerve react with the same monoclonal antibody. A fourth group of monoclonal antibodies, designated GLA, reacts with Bowman's glands and in some instances with secretory cells present in the respiratory mucosa. Two clones, BCL, stain at the level of the basal cell layer just above the lamina propria. A number of other antibodies react with cells and structures of the epithelium that have not been previously described. One of this group, NIS-1, stains globular structures present in the mucosa of the neuroepithelium.

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