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Journal of Neuroscience, Vol 5, 643-653, Copyright © 1985 by Society for Neuroscience
Monoclonal antibodies which distinguish certain classes of neuronal and supporting cells in the nervous tissue of the nematode Caenorhabditis elegans
H Okamoto and JN Thomson
Monoclonal antibodies were generated using mice immunized with total
homogenates of Caenorhabditis elegans adults or early larvae. Two of them
were shown to distinguish a certain class of neuronal or supporting cells
in the nervous tissue of this animal. Their histological specificities were
studied in detail by indirect immunofluorescence on a whole mount
preparation of animal head (or tail); for one of the antibodies further
analysis was done by immunoelectron microscopy with the aid of a colloidal
gold probe. An application of this antibody to a mutant of C. elegans is
also described.
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