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Journal of Neuroscience, Vol 5, 1886-1895, Copyright © 1985 by Society for Neuroscience
Optical mapping of electrical activity in rat somatosensory and visual cortex
HS Orbach, LB Cohen and A Grinvald
We have investigated the use of optical methods for monitoring neuron
activity in mammalian cortex. The cortex was stained with a voltage-
sensitive dye and fluorescence was simultaneously measured from 124 areas
using a photodiode array. Optical signals were detected in rat
somatosensory cortex in response to small whisker movements and in visual
cortex in response to light flashes to the eye. Relatively large signals
were obtained during focal interictal epileptiform discharges induced by
bicuculline. The measuring system had a time resolution of milliseconds and
a spatial resolution of a few hundred micrometers. Simultaneous, multi-site
optical recordings of activity may provide a new and potentially powerful
method for studying function and dysfunction in mammalian cortex.
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