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Journal of Neuroscience, Vol 5, 2028-2034, Copyright © 1985 by Society for Neuroscience
Differential synapse formation and neurite outgrowth at two branches of the metacerebral cell of Aplysia in dissociated cell culture
S Schacher
The metacerebral cell (MCC) of Aplysia californica was isolated with its
bifurcate axon from the cerebral ganglion and maintained in vitro under
three conditions: (a) with no targets, (b) with identified buccal ganglion
neurons B1 or B2 placed near the stump of the large diameter
cerebral-buccal connective (CBC) branch, and (c) with B1 or B2 placed near
the stump of the small diameter posterior lip nerve (PLN) branch. After 5
days in culture, the two branches differed significantly in the formation
of chemical connections and in the extent of neurite outgrowth. Chemical
connections characteristic of MCC-B1(B2) connections in vivo were observed
in more than 90% of the cultures in which the buccal neuron was contacted
by neurites emerging from the CBC branch, but in only 20% of the cultures
in which the buccal neuron was contacted by neurites extending from the PLN
branch. Neurite outgrowth from the CBC stump was always greater than growth
from the PLN and was not affected significantly by the presence of a buccal
neuron target at either branch. In contrast, neurite outgrowth from the PLN
decreased significantly when the target was contacted by neurites from the
CBC branch. These results suggest that two branches of a single neuron can
differ in their capacities to form chemical connections. In addition, the
two branches show differential growth as a result of target interaction at
one of the branches. This simple in vitro system may therefore be useful in
exploring the ways in which individual neurons control neurite extension
from different branches as they seek to form chemical connections with
their targets.
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