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Journal of Neuroscience, Vol 6, 2278-2289, Copyright © 1986 by Society for Neuroscience
Development and axonal outgrowth of identified motoneurons in the zebrafish
PZ Myers, JS Eisen and M Westerfield
We have observed the development of live, fluorescently labeled motoneurons
in the spinal cord of embryonic and larval zebrafish. There are 2 classes
of motoneurons: primary and secondary. On each side of each spinal segment
there are 3 individually identifiable primary motoneurons, named CaP, MiP,
and RoP. The motoneurons of the embryo and larva are similar in morphology
and projection pattern to those of the adult. During initial development,
axons of primary motoneurons make cell-specific, divergent pathway choices
and grow without error to targets appropriate for their adult functions. We
observed no period of cell death, and except for one consistently observed
case, there was no remodeling of peripheral arbors. We have observed a
consistent temporal sequence of axonal outgrowth within each spinal
segment. The CaP motor axon is the first to leave the spinal cord, followed
by the axons of the other primary motoneurons. The Mauthner growth cone
enters the spinal cord after all the primary motoneurons of the trunk
spinal cord have begun axonal outgrowth. Secondary motor growth cones
appear only after the Mauthner growth cone has passed by. Our results
suggest that this stereotyped temporal sequence of axonal outgrowth may
play a role in defining the contacts between the Mauthner axon and the
motoneurons; the behavior of growth cones in the periphery suggests that
interactions with the environment, not timing, may determine path- finding
and peripheral connectivity of the motoneurons.
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