QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Magistretti, P. J. Articles by Martin, J. L. Search for Related Content PubMed PubMed Citation Articles by Magistretti, P. J. Articles by Martin, J. L.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 6, 2558-2562, Copyright © 1986 by Society for Neuroscience

ARTICLE

Adenosine stimulates glycogenolysis in mouse cerebral cortex: a possible coupling mechanism between neuronal activity and energy metabolism

PJ Magistretti, PR Hof and JL Martin

Adenosine promotes a concentration-dependent hydrolysis of 3H-glycogen newly synthesized from 3H-glucose by mouse cerebral cortical slices. The EC50 for this effect is 7 microM. Theophylline antagonizes the glycogenolysis induced by adenosine with an EC50 of 80 microM. The rank- order of potencies of adenosine agonists is adenosine 5'-cyclopropyl- carboxamide greater than 2-chloroadenosine much greater than N6- cyclohexyladenosine = adenosine, suggesting that adenosine promotes glycogenolysis via receptors of the A2 type. This contention is substantiated by the weak stereospecificity observed for the glycogenolytic action of D- and L-(phenylisopropyl)-adenosine. The glycogenolysis elicited by adenosine at 10 and 100 microM is inhibited by ouabain at 10 microM, a concentration of the cardiac glycoside not significantly affecting 3H-glycogen levels per se. Interestingly, the previously demonstrated glycogenolytic action of vasoactive intestinal peptide (Magistretti et al., 1981, 1984) and of norepinephrine (Quach et al., 1978) is also antagonized by ouabain. These results demonstrate the existence of a metabolic action of adenosine, which is sensitive to ouabain and appears to be mediated by A2 receptors. The concentrations at which adenosine promotes glycogenolysis are of the same order of magnitude as those reached extracellularly by the nucleoside during neuronal depolarization (Pull and McIlwain, 1972). This set of observations therefore supports the notion that adenosine plays a modulatory role in the coupling between neuronal activity and energy metabolism in the CNS.

This article has been cited by other articles:

				I. Allaman, S. Lengacher, P. J. Magistretti, and L. Pellerin A2B receptor activation promotes glycogen synthesis in astrocytes through modulation of gene expression Am J Physiol Cell Physiol, March 1, 2003; 284(3): C696 - C704. [Abstract] [Full Text] [PDF]

				R. G. Boutilier Mechanisms of cell survival in hypoxia and hypothermia J. Exp. Biol., March 11, 2002; 204(18): 3171 - 3181. [Abstract] [Full Text] [PDF]

				P. Fox, M. Raichle, M. Mintun, and C Dence Nonoxidative glucose consumption during focal physiologic neural activity Science, July 22, 1988; 241(4864): 462 - 464. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help