 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 7, 3588-3599, Copyright © 1987 by Society for Neuroscience
Electrically and chemically mediated increases in intracellular calcium in neuronal growth cones
CS Cohan, JA Connor and SB Kater
Department of Biology, University of Iowa, Iowa City 52242.
In the present report we used the calcium indicator fura-2 to compare
intracellular levels of free calcium in growth cones of isolated Helisoma
neurons under a variety of experimental conditions. We tested whether 2
different signals that inhibit growth cone motility--action potentials and
serotonin--changed calcium levels in growth cones. Electrical stimulation
of the cell body caused a rise in calcium levels at the growth cone. After
brief stimulation, calcium levels quickly recovered to normal values,
whereas longer stimulation periods required longer recovery times. The
application of serotonin to growth cones caused an increase in calcium
levels that was selective for growth cones of neurons whose outgrowth was
inhibited by serotonin, but not for neurons whose outgrowth was not
affected. We also found that motile growth cones had higher free calcium
levels than growth cones that had spontaneously stopped growing.
Furthermore, the distribution of calcium in neurons that contained motile
growth cones was heterogeneous; calcium levels were always higher in the
growth cone than in the neurite or soma. These data indicate that calcium
levels in growth cones vary in different states of outgrowth and that
calcium levels can be modulated by both electrical and chemical signals.
This article has been cited by other articles:
|
|
|
|
 
X.-F. Zhang and P. Forscher
Rac1 Modulates Stimulus-evoked Ca2+ Release in Neuronal Growth Cones via Parallel Effects on Microtubule/Endoplasmic Reticulum Dynamics and Reactive Oxygen Species Production
Mol. Biol. Cell,
August 15, 2009;
20(16):
3700 - 3712.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hui, G.-H. Fei, B. J. Saab, J. Su, J. C. Roder, and Z.-P. Feng
Neuronal calcium sensor-1 modulation of optimal calcium level for neurite outgrowth
Development,
December 15, 2007;
134(24):
4479 - 4489.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I-F. Peng, B. A. Berke, Y. Zhu, W.-H. Lee, W. Chen, and C.-F. Wu
Temperature-Dependent Developmental Plasticity of Drosophila Neurons: Cell-Autonomous Roles of Membrane Excitability, Ca2+ Influx, and cAMP Signaling
J. Neurosci.,
November 14, 2007;
27(46):
12611 - 12622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S L Tam, V Archibald, N Tyreman, and T Gordon
Tetrodotoxin prevents motor unit enlargement after partial denervation in rat hindlimb muscles
J. Physiol.,
September 1, 2002;
543(2):
655 - 663.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F.-Q. Zhou, C. M. Waterman-Storer, and C. S. Cohan
Focal loss of actin bundles causes microtubule redistribution and growth cone turning
J. Cell Biol.,
May 28, 2002;
157(5):
839 - 849.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Duch and R. B. Levine
Changes in Calcium Signaling During Postembryonic Dendritic Growth in Manduca sexta
J Neurophysiol,
March 1, 2002;
87(3):
1415 - 1425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F.-q. Zhou and C. S. Cohan
Growth Cone Collapse through Coincident Loss of Actin Bundles and Leading Edge Actin without Actin Depolymerization
J. Cell Biol.,
May 28, 2001;
153(5):
1071 - 1084.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Zwingman, P. E. Neumann, J. L. Noebels, and K. Herrup
Rocker Is a New Variant of the Voltage-Dependent Calcium Channel Gene Cacna1a
J. Neurosci.,
February 15, 2001;
21(4):
1169 - 1178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Duch and R. B. Levine
Remodeling of Membrane Properties and Dendritic Architecture Accompanies the Postembryonic Conversion of a Slow into a Fast Motoneuron
J. Neurosci.,
September 15, 2000;
20(18):
6950 - 6961.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E M Fitzgerald
Regulation of voltage-dependent calcium channels in rat sensory neurones involves a Ras-mitogen-activated protein kinase pathway
J. Physiol.,
September 15, 2000;
527(3):
433 - 444.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. Diefenbach, P. B. Guthrie, and S. B. Kater
Stimulus History Alters Behavioral Responses of Neuronal Growth Cones
J. Neurosci.,
February 15, 2000;
20(4):
1484 - 1494.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Welnhofer, L. Zhao, and C. S. Cohan
Calcium Influx Alters Actin Bundle Dynamics and Retrograde Flow in Helisoma Growth Cones
J. Neurosci.,
September 15, 1999;
19(18):
7971 - 7982.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Hallows and B. L. Tempel
Expression of Kv1.1, a Shaker-Like Potassium Channel, Is Temporally Regulated in Embryonic Neurons and Glia
J. Neurosci.,
August 1, 1998;
18(15):
5682 - 5691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. A. Lnenicka, K. F. Arcaro, and J. M. Calabro
Activity-Dependent Development of Calcium Regulation in Growing Motor Axons
J. Neurosci.,
July 1, 1998;
18(13):
4966 - 4972.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Vance, C. M. Begg, W.-L. Lee, H. Haase, T. D. Copeland, and M. W. McEnery
Differential Expression and Association of Calcium Channel alpha 1B and beta Subunits during Rat Brain Ontogeny
J. Biol. Chem.,
June 5, 1998;
273(23):
14495 - 14502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. L. Herring, I. M. Slotin, J. M. Baltz, and C. E. Morris
Neuronal swelling and surface area regulation: elevated intracellular calcium is not a requirement
Am J Physiol Cell Physiol,
January 1, 1998;
274(1):
C272 - C281.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Koulen, R. Kuhn, H. Wassle, and J. H. Brandstatter
Group I Metabotropic Glutamate Receptors mGluR1alpha and mGluR5a: Localization in Both Synaptic Layers of the Rat Retina
J. Neurosci.,
March 15, 1997;
17(6):
2200 - 2211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Shitaka, N. Matsuki, H. Saito, and H. Katsuki
Basic Fibroblast Growth Factor Increases Functional L-Type Ca2+ Channels in Fetal Rat Hippocampal Neurons: Implications for Neurite Morphogenesis In Vitro
J. Neurosci.,
October 15, 1996;
16(20):
6476 - 6489.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Morris and P. Hollenbeck
The regulation of bidirectional mitochondrial transport is coordinated with axonal outgrowth
J. Cell Sci.,
January 3, 1993;
104(3):
917 - 927.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. Bandtlow, M. Schmidt, T. Hassinger, M. Schwab, and S. Kater
Role of intracellular calcium in NI-35-evoked collapse of neuronal growth cones
Science,
January 1, 1993;
259(5091):
80 - 83.
[Abstract]
[PDF]
|
|
|
|
|
|
|
E. Dreyer, P. Kaiser, J. Offermann, and S. Lipton
HIV-1 coat protein neurotoxicity prevented by calcium channel antagonists
Science,
April 20, 1990;
248(4953):
364 - 367.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S.B. Kater and P.B. Guthrie
Neuronal Growth Cone as an Integrator of Complex Environmental Information
Cold Spring Harb Symp Quant Biol,
January 1, 1990;
55(0):
359 - 370.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. Morris and W. Sigurdson
Stretch-inactivated ion channels coexist with stretch-activated ion channels
Science,
February 10, 1989;
243(4892):
807 - 809.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Connor, W. Wadman, P. Hockberger, and R. Wong
Sustained dendritic gradients of Ca2+ induced by excitatory amino acids in CA1 hippocampal neurons
Science,
April 29, 1988;
240(4852):
649 - 653.
[Abstract]
[PDF]
|
|
|
|
|
