 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 7, 3712-3722, Copyright © 1987 by Society for Neuroscience
Nondirected axonal growth on basal lamina from avian embryonic neural retina
W Halfter, W Reckhaus and S Kroger
Max-Planck-Institut fur Entwicklungsbiologie, Tubingen, FRG.
The vitreous surface of the embryonic avian retinal neuroepithelium was
isolated by mechanical disruption of the retina mounted between 2 adhesive
substrata. The 200-micron-thick sheath covered an area of up to 1 cm2 and
consisted of the vitreal basal lamina with a lamina densa, 2 laminae rarae,
and a carpet of ventricular cell endfeet on top of the lamina. The vitreal
endfeet were removed by detergent treatment and an extracellular basal
lamina was obtained. The laminae were further characterized by
immunohistochemistry and immunoblotting. A 190 kDa laminin protein was
detected in laminae with and without vitreal endfeet, whereas the
membrane-bound neural cell adhesion molecule (N- CAM) was detectable only
on the endfeet of the ventricular cells and was absent in the
detergent-treated basal laminae. Neither immunoblotting nor immunostaining
revealed fibronectin in these preparations. Explants of retina, sensory
ganglia, and cerebellum from chick, quail, and mouse were cultured on the
basal lamina as a substratum. In all cases axonal outgrowth was excellent,
with a growth rate similar to that in situ. Outgrowing axons from sensory
ganglia and cerebellar explants were accompanied by migratory cells, which,
in the case of sensory ganglia, were flat cells and, in the case of
cerebellar explants, resembled granular neurons. Optic axons grew on the
laminae in an asymmetric, explant-inherent pattern specific for the
position of origin of the explant. On detergent-treated basal laminae, as
well as on laminin, the retinal axons grew in a clockwise orientation. This
axonal growth pattern was specific for retinal tissue and was not observed
with axons from other neural explants. In spite of the excellent substrate
properties provided by the substratum, cues for growing axons (toward or
away from the optic disk) were not detectable in the basal lamina
preparations.
This article has been cited by other articles:
|
|
|
|
 
R. Ramseger, R. White, and S. Kroger
Transmembrane Form Agrin-induced Process Formation Requires Lipid Rafts and the Activation of Fyn and MAPK
J. Biol. Chem.,
March 20, 2009;
284(12):
7697 - 7705.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Halfter, M. Willem, and U. Mayer
Basement Membrane-Dependent Survival of Retinal Ganglion Cells
Invest. Ophthalmol. Vis. Sci.,
March 1, 2005;
46(3):
1000 - 1009.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Freire, F. C. A. Gomes, R. Linden, V. M. Neto, and T. Coelho-Sampaio
Structure of laminin substrate modulates cellular signaling for neuritogenesis
J. Cell Sci.,
March 14, 2003;
115(24):
4867 - 4876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Dong, G. J. Cole, and W. Halfter
Expression of Collagen XVIII and Localization of Its Glycosaminoglycan Attachment Sites
J. Biol. Chem.,
January 10, 2003;
278(3):
1700 - 1707.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Stepanek, Q. L. Sun, J. Wang, C. Wang, and J. L. Bixby
CRYP-2/cPTPRO is a neurite inhibitory repulsive guidance cue for retinal neurons in vitro
J. Cell Biol.,
August 20, 2001;
154(4):
867 - 878.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Ledig, F. Haj, J. L. Bixby, A. W. Stoker, and B. K. Mueller
The Receptor Tyrosine Phosphatase Cryp{alpha} Promotes Intraretinal Axon Growth
J. Cell Biol.,
October 18, 1999;
147(2):
375 - 388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Halfter, S. Dong, B. Schurer, and G. J. Cole
Collagen XVIII Is a Basement Membrane Heparan Sulfate Proteoglycan
J. Biol. Chem.,
September 25, 1998;
273(39):
25404 - 25412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Bauch, H. Stier, and B. Schlosshauer
Axonal Versus Dendritic Outgrowth Is Differentially Affected by Radial Glia in Discrete Layers of the Retina
J. Neurosci.,
March 1, 1998;
18(5):
1774 - 1785.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Halfter
The Behavior of Optic Axons on Substrate Gradients of Retinal Basal Lamina Proteins and Merosin
J. Neurosci.,
July 15, 1996;
16(14):
4389 - 4401.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H Stier and B Schlosshauer
Axonal guidance in the chicken retina
Development,
January 5, 1995;
121(5):
1443 - 1454.
[Abstract]
[PDF]
|
|
|
|
|
|
|
P. Brittis, D. Canning, and J Silver
Chondroitin sulfate as a regulator of neuronal patterning in the retina
Science,
February 7, 1992;
255(5045):
733 - 736.
[Abstract]
[PDF]
|
|
|
|
|
|
|
U.J. McMahan
The Agrin Hypothesis
Cold Spring Harb Symp Quant Biol,
January 1, 1990;
55(0):
407 - 418.
[Abstract]
[PDF]
|
|
|
|
|
