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Journal of Neuroscience, Vol 7, 418-431, Copyright © 1987 by Society for Neuroscience
Voltage-dependent gating of single sodium channels from mammalian neuroblastoma cells
RW Aldrich and CF Stevens
Single sodium channel currents have been studied in cell-attached patches
from the mouse neuroblastoma cell line N1E115. Distributions of open
duration, latency until first opening, and the average probability of a
channel being open after a voltage step, p(t), were analyzed and compared
to predicted distributions from various kinetic models for
voltage-dependent gating. It was found that, over most of the voltage range
under which channel gating occurs, the slow steps in gating are opening
transitions and that inactivation of open channels is significantly faster
than the decline in p(t) (tau h). This view of gating is confirmed by
comparison of the kinetics of ensemble averages of single-channel currents
obtained from step- and tail-current records at the same voltage. The
probability of a channel reopening after having closed was calculated by
comparing p(t) with the convolution of the first-latency probability
density and the conditional probability of remaining open t milliseconds
after opening. This reopening probability is small but slightly voltage
dependent over the voltage range where the mean open duration remains
constant and tau h changes considerably. The voltage dependence of open
channel inactivation and deactivation were calculated from the probability
of reopening and the mean open duration. The equivalent gating charge for
the inactivation rate is a few tenths of an electronic charge, whereas the
equivalent charge for the closing rate is 2.5-3.5 electronic charges.
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