Journal of Neuroscience, Vol 7, 512-521, Copyright © 1987 by Society for Neuroscience
Immunological probes reveal spatial and developmental diversity in insect neuroglia
MR Meyer, GR Reddy and JS Edwards
A set of monoclonal antibodies (MAbs) has been generated that recognizes
distinct classes of neuroglia in the adult nervous system of the cricket
Acheta domesticus corresponding to glial types distinguished by
morphological criteria. These include antibodies that bind to the neuroglia
of the ganglionic cortex, perineurium, neuropil, and glia associated with
the glial lacunar system (interface) and fiber tracts. Another MAb
specifically labels components of the neural lamella, a complex
extracellular matrix secreted by underlying perineurial cells. Selected
adult glial-specific MAbs recognize particular glial antigens expressed
during embryonic development of Acheta. Immunohistochemical staining of
frozen sections of late- (90- 95%) and intermediate- (50-55%) stage whole
embryos reveals that the spatial distribution, degree of tissue
restriction, or level of expression of some glial determinants changes as
development proceeds. Labeling of certain neuroblasts in the embryonic CNS
at 50-55% development by an antibody (MAb 3G6) that binds to neuropil glia
in the adult CNS implies that at least 1 class of insect glia may be
generated by these cells.