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 Previous Article
Journal of Neuroscience, Vol 7, 1928-1934, Copyright © 1987 by Society for Neuroscience
Glial-guided granule neuron migration in vitro: a high-resolution time- lapse video microscopic study
JC Edmondson and ME Hatten
To study neuronal migration, migrating granule neurons in microcultures
prepared from early postnatal cerebellum have been analyzed with time-
lapse, video-enhanced differential interference contrast microscopy. The
morphology of migrating neurons resembles the elongated forms of migrating
neurons described both in vivo and in vitro (Rakic, 1971; Hatten et al.,
1984). The neuron closely apposes its soma along the glial fiber and
extends a thickened leading process in the direction of migration. This
leading tip is highly motile, with several filopodial extensions.
Intracellular vesicular structures extend from the nucleus into the leading
process of migrating neurons in vitro. Quantitation of the motions of
migrating neurons revealed a saltatory pattern of advance along the glial
fiber. Periods of cell soma movement at the rate of 56 +/- 26 micron/hr
along the glial fiber are punctuated by periods during which the cell soma
slows to a complete stop. The overall rate of migration is 33 +/- 20
micron/hr. The growing tip of the leading process rapidly extends and
retracts, resulting in a net advance along the glial fiber. However, the
periods of the extension and retraction of the leading process growing tip
are not synchronized with the motions of the cell soma.
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