WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience MBF Bioscience Autoneuron
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xie, G. X.
Right arrow Articles by Goldstein, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xie, G. X.
Right arrow Articles by Goldstein, A.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 7, 2049-2055, Copyright © 1987 by Society for Neuroscience

ARTICLE

Characterization of big dynorphins from rat brain and spinal cord

GX Xie and A Goldstein

To examine the processing of products of the dynorphin gene in the central nervous system, immunoreactive (ir) dynorphin (Dyn) A, Dyn B, Dyn A-(1-8), alpha- and beta-neo-endorphin (alpha- and beta-Neo) in rat brain and spinal cord were measured, using specific antisera after gel filtration and high-performance liquid chromatography (HPLC). Three peaks of Mr about 8, 4, and 2 kDa for ir-Dyn A and ir-Dyn B, and one peak of Mr less than 2 kDa for ir-Dyn A-(1-8), ir-alpha-, and ir-beta- Neo were found both in the brain and in the spinal cord. The 8 kDa peak was recognized by Dyn A and Dyn B antisera and, after hydrolysis by proline-specific endopeptidase, by beta-Neo antiserum. The 8 kDa peak was recognized by a monoclonal antibody against the amino terminal sequence Tyr-Gly-Gly-Phe of all opioid peptides and by an antiserum directed toward the carboxyl terminus of Dyn B, indicating that it contains, from the amino terminal tyrosine of neo-endorphin to the carboxyl-terminal threonine of Dyn B, all 3 opioid peptide regions in the prodynorphin. By means of proline-specific endopeptidase hydrolysis, we also found a big dynorphin precursor (Mr approximately equal to 26 kDa) in both brain and spinal cord.


This article has been cited by other articles:


Home page
 Circ. Res.Home page
C. Ventura, E. Zinellu, E. Maninchedda, and M. Maioli
Dynorphin B Is an Agonist of Nuclear Opioid Receptors Coupling Nuclear Protein Kinase C Activation to the Transcription of Cardiogenic Genes in GTR1 Embryonic Stem Cells
Circ. Res., April 4, 2003; 92(6): 623 - 629.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. Boudarine, O. Yegorov, A. Sterling-Dubrovsky, L. A. Devi, and Y. Berman
Developmental Changes in Opioid Peptides and Their Receptors in Cpefat/Cpefat Mice Lacking Peptide Processing Enzyme Carboxypeptidase E
J. Pharmacol. Exp. Ther., December 1, 2002; 303(3): 1317 - 1324.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Ventura, G. Pintus, M. G. Fiori, F. Bennardini, G. Pinna, and L. Gaspa
Opioid Peptide Gene Expression in the Primary Hereditary Cardiomyopathy of the Syrian Hamster. I. REGULATION OF PRODYNORPHIN GENE EXPRESSION BY NUCLEAR PROTEIN KINASE C
J. Biol. Chem., March 7, 1997; 272(10): 6685 - 6692.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Ventura, G. Pintus, I. Vaona, F. Bennardini, G. Pinna, and B. Tadolini
Phorbol Ester Regulation of Opioid Peptide Gene Expression in Myocardial Cells
J. Biol. Chem., December 15, 1995; 270(50): 30115 - 30120.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-