 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 7, 2711-2720, Copyright © 1987 by Society for Neuroscience
Light and electron microscopic localization of a cell surface antigen (NG2) in the rat cerebellum: association with smooth protoplasmic astrocytes
JM Levine and JP Card
Immunofluorescence and immunoperoxidase techniques were used to localize a
cell surface chondroitin-sulfate proteoglycan antigen, termed NG2, in the
developing and adult rat cerebellum. In the adult, both polyclonal and
monoclonal anti-NG2 antibodies labeled cells throughout the cerebellar
cortex, with the labeled cells being especially prominent in the molecular
layer. The labeled cells had small, irregularly shaped cell bodies from
which thin highly branched processes radiated in a stellate array. The
NG2-labeled cells were not labeled with antibodies against glial fibrillary
acidic protein (GFAP), vimentin, or S-100 protein, intracellular markers
for astrocytes. However, electron microscopic immunocytochemical analysis
of NG2 immunoreactive cells revealed a cell morphology consistent with that
of protoplasmic astrocytes. Labeled cell bodies contained a thin rim of
organelle-poor cytoplasm surrounding a euchromatic nucleus. Thick processes
originating from the cell soma tapered to form thin branches with highly
irregular surface contours that extended between adjacent neuronal
elements. The labeled processes did not form synapses in the neuropil, and
no synaptic profiles onto anti-NG2-labeled cell bodies or processes were
observed. Thus, we conclude that the NG2 antigen is a cell surface marker
for a class of smooth protoplasmic astrocytes. Immunoreactive cells were
seen in the developing cerebellum beginning at embryonic day 16. The number
of labeled cells increased during the early stages of cerebellar
development, reaching a peak at about postnatal day (PND) 4 or 5 and
declining thereafter. In the developing cerebellum, labeled cells lying
within the forming molecular layer resembled the cells seen in the adult,
whereas cells lying deeper within the folia had an immature appearance with
fewer processes and less branching. This apparent gradient of morphological
maturation suggests that an interaction with parallel fibers in the
developing molecular layer may play a role in the terminal
cytodifferentiation of the NG2-labeled smooth protoplasmic astrocytes.
This article has been cited by other articles:
|
|
|
|
 
A. Nishiyama
Polydendrocytes: NG2 Cells with Many Roles in Development and Repair of the CNS
Neuroscientist,
February 1, 2007;
13(1):
62 - 76.
[Abstract]
[PDF]
|
|
|
|
|
|
|
W.-P. Ge, X.-J. Yang, Z. Zhang, H.-K. Wang, W. Shen, Q.-D. Deng, and S. Duan
Long-term potentiation of neuron-glia synapses mediated by Ca2+-permeable AMPA receptors.
Science,
June 9, 2006;
312(5779):
1533 - 1537.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Ligon, S. Kesari, M. Kitada, T. Sun, H. A. Arnett, J. A. Alberta, D. J. Anderson, C. D. Stiles, and D. H. Rowitch
Development of NG2 neural progenitor cells requires Olig gene function
PNAS,
May 16, 2006;
103(20):
7853 - 7858.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Zhou, G. P. Schools, and H. K. Kimelberg
Development of GLAST(+) Astrocytes and NG2(+) Glia in Rat Hippocampus CA1: Mature Astrocytes Are Electrophysiologically Passive
J Neurophysiol,
January 1, 2006;
95(1):
134 - 143.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. Huang, G. R. Phillips, A. D. Roth, L. Pedraza, W. Shan, W. Belkaid, S. Mi, A. Fex-Svenningsen, L. Florens, J. R. Yates III, et al.
Glial Membranes at the Node of Ranvier Prevent Neurite Outgrowth
Science,
December 16, 2005;
310(5755):
1813 - 1817.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Smyth and P. Scambler
The genetics of Fraser syndrome and the blebs mouse mutants
Hum. Mol. Genet.,
October 15, 2005;
14(suppl_2):
R269 - R274.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Peters and C. Sethares
Oligodendrocytes, their Progenitors and other Neuroglial Cells in the Aging Primate Cerebral Cortex
Cereb Cortex,
September 1, 2004;
14(9):
995 - 1007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. M. Ughrin, Z. J. Chen, and J. M. Levine
Multiple Regions of the NG2 Proteoglycan Inhibit Neurite Growth and Induce Growth Cone Collapse
J. Neurosci.,
January 1, 2003;
23(1):
175 - 186.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Martin, A. K. Levine, Z. J. Chen, Y. Ughrin, and J. M. Levine
Deposition of the NG2 Proteoglycan at Nodes of Ranvier in the Peripheral Nervous System
J. Neurosci.,
October 15, 2001;
21(20):
8119 - 8128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Tang, Y. M. Tokumoto, and M. C. Raff
Long-Term Culture of Purified Postnatal Oligodendrocyte Precursor Cells: Evidence for an Intrinsic Maturation Program that Plays out over Months
J. Cell Biol.,
March 6, 2000;
148(5):
971 - 984.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Shi, A. Marinovich, and B. A. Barres
Purification and Characterization of Adult Oligodendrocyte Precursor Cells from the Rat Optic Nerve
J. Neurosci.,
June 15, 1998;
18(12):
4627 - 4636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
