 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 7, 2737-2744, Copyright © 1987 by Society for Neuroscience
Bipotential glial precursor cells of the optic nerve express the NG2 proteoglycan
WB Stallcup and L Beasley
We have studied the expression of the NG2 chondroitin sulfate proteoglycan
on bipotential glial precursor cells in cultures of postnatal rat optic
nerve. Purified populations of these precursor cells were prepared by
panning dissociated optic nerve cells on dishes coated with monoclonal A2B5
antibody. Using immunofluorescence double staining, we found that NG2 was
present on almost 95% of the purified A2B5+ precursor cells. The NG2 core
protein from optic nerve cells was identified by immune precipitation and
PAGE and was found to be identical to the 300,000 Da NG2 core protein from
a clonal rat cell line B49. Over a culture period of 5 d in medium
containing 10% fetal calf serum, more than 80% of the NG2+ precursor cells
acquired the glial fibrillary acidic protein (GFAP), an astrocyte-specific
marker. Under these conditions, fewer than 10% of the NG2+ cells expressed
galactocerebroside (GC), an oligodendrocyte-specific marker. These GFAP+GC-
type II astrocytes continued to express the NG2 antigen for up to 10 d in
culture. During a 5 d culture period in hormonally supplemented, serum-free
medium, fewer than 15% of the NG2+ cells expressed GFAP, while up to 40%
expressed GC. The NG2 antigen continued to be expressed for only a short
period of time by these GFAP-GC+ oligodendrocytes, so that mature
oligodendrocytes in the cultures became NG2-. These results support our
previous suggestion that the NG2 antigen is found on a class of neural
cells that can differentiate along more than one pathway.
This article has been cited by other articles:
|
|
|
|
 
V. Gallo, J.-M. Mangin, M. Kukley, and D. Dietrich
Synapses on NG2-expressing progenitors in the brain: multiple functions?
J. Physiol.,
August 15, 2008;
586(16):
3767 - 3781.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ferletta, L. Uhrbom, T. Olofsson, F. Ponten, and B. Westermark
Sox10 Has a Broad Expression Pattern in Gliomas and Enhances Platelet-Derived Growth Factor-B Induced Gliomagenesis
Mol. Cancer Res.,
September 1, 2007;
5(9):
891 - 897.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Yanagisawa and R. K Yu
The expression and functions of glycoconjugates in neural stem cells
Glycobiology,
July 1, 2007;
17(7):
57R - 74R.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Hayakawa-Yano, K. Nishida, S. Fukami, Y. Gotoh, T. Hirano, T. Nakagawa, T. Shimazaki, and H. Okano
Epidermal Growth Factor Signaling Mediated by Grb2 Associated Binder1 Is Required for the Spatiotemporally Regulated Proliferation of Olig2-Expressing Progenitors in the Embryonic Spinal Cord
Stem Cells,
June 1, 2007;
25(6):
1410 - 1422.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nishiyama
Polydendrocytes: NG2 Cells with Many Roles in Development and Repair of the CNS
Neuroscientist,
February 1, 2007;
13(1):
62 - 76.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Z. Yang, R. Suzuki, S. B. Daniels, C. B. Brunquell, C. J. Sala, and A. Nishiyama
NG2 glial cells provide a favorable substrate for growing axons.
J. Neurosci.,
April 5, 2006;
26(14):
3829 - 3839.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Jakovcevski and N. Zecevic
Olig Transcription Factors Are Expressed in Oligodendrocyte and Neuronal Cells in Human Fetal CNS
J. Neurosci.,
November 2, 2005;
25(44):
10064 - 10073.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Kessaris, F. Jamen, L. L. Rubin, and W. D. Richardson
Cooperation between sonic hedgehog and fibroblast growth factor/MAPK signalling pathways in neocortical precursors
Development,
March 15, 2004;
131(6):
1289 - 1298.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Ye, R. Bagnell, and A. J. D'Ercole
Mouse NG2+ Oligodendrocyte Precursors Express mRNA for Proteolipid Protein But Not Its DM-20 Variant: A Study of Laser Microdissection-Captured NG2+ Cells
J. Neurosci.,
June 1, 2003;
23(11):
4401 - 4405.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Jarjour, C. Manitt, S. W. Moore, K. M. Thompson, S.-J. Yuh, and T. E. Kennedy
Netrin-1 Is a Chemorepellent for Oligodendrocyte Precursor Cells in the Embryonic Spinal Cord
J. Neurosci.,
May 1, 2003;
23(9):
3735 - 3744.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Belachew, R. Chittajallu, A. A. Aguirre, X. Yuan, M. Kirby, S. Anderson, and V. Gallo
Postnatal NG2 proteoglycan-expressing progenitor cells are intrinsically multipotent and generate functional neurons
J. Cell Biol.,
April 14, 2003;
161(1):
169 - 186.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Stegmuller, H. Werner, K.-A. Nave, and J. Trotter
The Proteoglycan NG2 Is Complexed with alpha -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors by the PDZ Glutamate Receptor Interaction Protein (GRIP) in Glial Progenitor Cells. IMPLICATIONS FOR GLIAL-NEURONAL SIGNALING
J. Biol. Chem.,
January 31, 2003;
278(6):
3590 - 3598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. P. Pringle, W.-P. Yu, M. Howell, J. S. Colvin, D. M. Ornitz, and W. D. Richardson
Fgfr3 expression by astrocytes and their precursors: evidence that astrocytes and oligodendrocytes originate in distinct neuroepithelial domains
Development,
January 1, 2003;
130(1):
93 - 102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Konishi, K. Lindholm, L.-B. Yang, R. Li, and Y. Shen
Isolation of Living Neurons from Human Elderly Brains Using the Immunomagnetic Sorting DNA-Linker System
Am. J. Pathol.,
November 1, 2002;
161(5):
1567 - 1576.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Gregori, C. Proschel, M. Noble, and M. Mayer-Proschel
The Tripotential Glial-Restricted Precursor (GRP) Cell and Glial Development in the Spinal Cord: Generation of Bipotential Oligodendrocyte-Type-2 Astrocyte Progenitor Cells and Dorsal-Ventral Differences in GRP Cell Function
J. Neurosci.,
January 1, 2002;
22(1):
248 - 256.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Sugimoto, M. Taniguchi, T. Yagi, Y. Akagi, Y. Nojyo, and N. Tamamaki
Guidance of glial precursor cell migration by secreted cues in the developing optic nerve
Development,
September 1, 2001;
128(17):
3321 - 3330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Dai, J. C. Celestino, Y. Okada, D. N. Louis, G. N. Fuller, and E. C. Holland
PDGF autocrine stimulation dedifferentiates cultured astrocytes and induces oligodendrogliomas and oligoastrocytomas from neural progenitors and astrocytes in vivo
Genes & Dev.,
August 1, 2001;
15(15):
1913 - 1925.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Tekki-Kessaris, R. Woodruff, A. C. Hall, W. Gaffield, S. Kimura, C. D. Stiles, D. H. Rowitch, and W. D. Richardson
Hedgehog-dependent oligodendrocyte lineage specification in the telencephalon
Development,
July 1, 2001;
128(13):
2545 - 2554.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. McTigue, P. Wei, and B. T. Stokes
Proliferation of NG2-Positive Cells and Altered Oligodendrocyte Numbers in the Contused Rat Spinal Cord
J. Neurosci.,
May 15, 2001;
21(10):
3392 - 3400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Kenney and D. H. Rowitch
Sonic hedgehog Promotes G1 Cyclin Expression and Sustained Cell Cycle Progression in Mammalian Neuronal Precursors
Mol. Cell. Biol.,
December 1, 2000;
20(23):
9055 - 9067.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. S. Shihabuddin, P. J. Horner, J. Ray, and F. H. Gage
Adult Spinal Cord Stem Cells Generate Neurons after Transplantation in the Adult Dentate Gyrus
J. Neurosci.,
December 1, 2000;
20(23):
8727 - 8735.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. E. Bandtlow and D. R. Zimmermann
Proteoglycans in the Developing Brain: New Conceptual Insights for Old Proteins
Physiol Rev,
October 1, 2000;
80(4):
1267 - 1290.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Chang, A. Nishiyama, J. Peterson, J. Prineas, and B. D. Trapp
NG2-Positive Oligodendrocyte Progenitor Cells in Adult Human Brain and Multiple Sclerosis Lesions
J. Neurosci.,
September 1, 2000;
20(17):
6404 - 6412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. McKeon, M. J. Jurynec, and C. R. Buck
The Chondroitin Sulfate Proteoglycans Neurocan and Phosphacan Are Expressed by Reactive Astrocytes in the Chronic CNS Glial Scar
J. Neurosci.,
December 15, 1999;
19(24):
10778 - 10788.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Ghiani, X. Yuan, A. M. Eisen, P. L. Knutson, R. A. DePinho, C. J. McBain, and V. Gallo
Voltage-Activated K+ Channels and Membrane Depolarization Regulate Accumulation of the Cyclin-Dependent Kinase Inhibitors p27Kip1 and p21CIP1 in Glial Progenitor Cells
J. Neurosci.,
July 1, 1999;
19(13):
5380 - 5392.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Niehaus, J. Stegmuller, M. Diers-Fenger, and J. Trotter
Cell-Surface Glycoprotein of Oligodendrocyte Progenitors Involved in Migration
J. Neurosci.,
June 15, 1999;
19(12):
4948 - 4961.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Goretzki, M. A. Burg, K. A. Grako, and W. B. Stallcup
High-affinity Binding of Basic Fibroblast Growth Factor and Platelet-derived Growth Factor-AA to the Core Protein of the NG2 Proteoglycan
J. Biol. Chem.,
June 11, 1999;
274(24):
16831 - 16837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ghiani, A. Eisen, X Yuan, R. DePinho, C. McBain, and V Gallo
Neurotransmitter receptor activation triggers p27(Kip1 )and p21(CIP1) accumulation and G1 cell cycle arrest in oligodendrocyte progenitors
Development,
January 2, 1999;
126(5):
1077 - 1090.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. Robinson, M. Tani, R. M. Strieter, R. M. Ransohoff, and R. H. Miller
The Chemokine Growth-Regulated Oncogene-alpha Promotes Spinal Cord Oligodendrocyte Precursor Proliferation
J. Neurosci.,
December 15, 1998;
18(24):
10457 - 10463.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Shi, A. Marinovich, and B. A. Barres
Purification and Characterization of Adult Oligodendrocyte Precursor Cells from the Rat Optic Nerve
J. Neurosci.,
June 15, 1998;
18(12):
4627 - 4636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-i. Sakakibara and H. Okano
Expression of Neural RNA-Binding Proteins in the Postnatal CNS: Implications of Their Roles in Neuronal and Glial Cell Development
J. Neurosci.,
November 1, 1997;
17(21):
8300 - 8312.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Tillet, F. Ruggiero, A. Nishiyama, and W. B. Stallcup
The Membrane-spanning Proteoglycan NG2 Binds to Collagens V and VI through the Central Nonglobular Domain of Its Core Protein
J. Biol. Chem.,
April 18, 1997;
272(16):
10769 - 10776.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Burg, E. Tillet, R. Timpl, and W. B. Stallcup
Binding of the NG2 Proteoglycan to Type VI Collagen and Other Extracellular Matrix Molecules
J. Biol. Chem.,
October 18, 1996;
271(42):
26110 - 26116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A Hall, N. Giese, and W. Richardson
Spinal cord oligodendrocytes develop from ventrally derived progenitor cells that express PDGF alpha-receptors
Development,
January 12, 1996;
122(12):
4085 - 4094.
[Abstract]
[PDF]
|
|
|
|
|
|
|
L. Smith-Thomas, J Fok-Seang, J Stevens, J. Du, E Muir, A Faissner, H. Geller, J. Rogers, and J. Fawcett
An inhibitor of neurite outgrowth produced by astrocytes
J. Cell Sci.,
January 6, 1994;
107(6):
1687 - 1695.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Raff
Glial cell diversification in the rat optic nerve
Science,
March 17, 1989;
243(4897):
1450 - 1455.
[Abstract]
[PDF]
|
|
|
|
