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Journal of Neuroscience, Vol 7, 2737-2744, Copyright © 1987 by Society for Neuroscience
Bipotential glial precursor cells of the optic nerve express the NG2 proteoglycan
WB Stallcup and L Beasley
We have studied the expression of the NG2 chondroitin sulfate proteoglycan
on bipotential glial precursor cells in cultures of postnatal rat optic
nerve. Purified populations of these precursor cells were prepared by
panning dissociated optic nerve cells on dishes coated with monoclonal A2B5
antibody. Using immunofluorescence double staining, we found that NG2 was
present on almost 95% of the purified A2B5+ precursor cells. The NG2 core
protein from optic nerve cells was identified by immune precipitation and
PAGE and was found to be identical to the 300,000 Da NG2 core protein from
a clonal rat cell line B49. Over a culture period of 5 d in medium
containing 10% fetal calf serum, more than 80% of the NG2+ precursor cells
acquired the glial fibrillary acidic protein (GFAP), an astrocyte-specific
marker. Under these conditions, fewer than 10% of the NG2+ cells expressed
galactocerebroside (GC), an oligodendrocyte-specific marker. These GFAP+GC-
type II astrocytes continued to express the NG2 antigen for up to 10 d in
culture. During a 5 d culture period in hormonally supplemented, serum-free
medium, fewer than 15% of the NG2+ cells expressed GFAP, while up to 40%
expressed GC. The NG2 antigen continued to be expressed for only a short
period of time by these GFAP-GC+ oligodendrocytes, so that mature
oligodendrocytes in the cultures became NG2-. These results support our
previous suggestion that the NG2 antigen is found on a class of neural
cells that can differentiate along more than one pathway.
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