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Journal of Neuroscience, Vol 8, 176-184, Copyright © 1988 by Society for Neuroscience
Protein-synthetic machinery beneath postsynaptic sites on CNS neurons: association between polyribosomes and other organelles at the synaptic site
O Steward and TM Reeves
Department of Neuroscience, University of Virginia School of Medicine, Charlottesville 22908.
Previous studies have demonstrated that polyribosomes are selectively
positioned beneath postsynaptic sites on CNS neurons. In spine-bearing
neurons, these polyribosomes are selectively localized at the base of the
spines, and occasionally within spine heads. The present study evaluates
whether there are relationships between the polyribosomes and other
organelles of the postsynaptic cytoplasm, including membranous cisterns and
spine apparatuses. Dendritic spines from the dentate gyrus and hippocampus
of the rat were analyzed at the electron-microscopic level in 2 ways.
First, relatively thick sections were prepared for electron microscopy, and
spines were photographed in stereo using a goniometer stage. Second,
conventional serial thin sections were taken, and spines were
reconstructed. From the stereo photographs and serial reconstructions, we
determined the proportion of polyribosomes that was associated with
membranous cisterns. We also counted the number of ribosomes per cluster to
determine whether there were differences between polyribosomes in different
intradendritic locations, or between free polyribosomes and polyribosomes
on cisternal membranes. From the serially reconstructed spines we
determined the incidence of polyribosomes, membranous cisterns, and spine
apparatuses, and evaluated the relationships between these organelles. We
found that in both the dentate gyrus and hippocampus, about 50% of the
polyribosomes that were present beneath the base of spines were associated
with membranous cisterns. Polyribosomes that were present in the head of
the spine were rarely associated with a cistern, however. The overall
incidence of polyribosomes was similar in spines with spine apparatuses and
spines without a spine apparatus.(ABSTRACT TRUNCATED AT 250 WORDS)
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