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Journal of Neuroscience, Vol 8, 3633-3643, Copyright © 1988 by Society for Neuroscience
Voltage-clamp analysis of sodium channels in wild-type and mutant Drosophila neurons
DK O'Dowd and RW Aldrich
Department of Neurobiology, Stanford University School of Medicine, California 94305.
In this study we describe a preparation in which we examined directly,
using tight-seal whole-cell recording, sodium currents from embryonic
Drosophila neurons maintained in culture. Sodium currents were expressed in
approximately 65% of the neurons prepared from wild-type Drosophila embryos
when examined at room temperature, 24 hr after plating. While current
density was low, other features of the sodium current in wild-type neurons,
including the voltage sensitivity, steady- state inactivation, macroscopic
time course, and TTX sensitivity were similar to those found in other
excitable cells. Physiological and biochemical evidence has led to the
suggestion that mutations in the nap, seizure, and tip-E loci of Drosophila
may affect voltage-dependent sodium channels. There was no significant
difference in the percentage of neurons expressing sodium currents in
cultures prepared from embryos with mutations at the nap, sei or tip-E loci
compared with wild-type cultures. Sodium currents recorded from napts
appeared similar in all of the properties examined to those in wild-type
cells. However, neuronal sodium current density was 40-60% lower in
cultures prepared from both tip-E and seits1 embryos. The voltage
dependence and gating properties of these sodium channels, as well as the
TTX sensitivity, appear similar to wild type. These results indicate that
both the tip-E and sei loci are important in regulation of sodium current
density in embryonic neurons.
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