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Journal of Neuroscience, Vol 8, 3722-3732, Copyright © 1988 by Society for Neuroscience
Development of inward currents in chick sensory and autonomic neuronal precursor cells in culture
K Gottmann, ID Dietzel, HD Lux, S Huck and H Rohrer
Department of Neurophysiology, Max Planck Institute for Psychiatry, Planegg-Martinsried, FRG.
The development of ionic inward currents was studied in cultured neuronal
precursors from chick sensory dorsal root ganglia (DRG) and compared with
neuronal precursors from the cholinergic ciliary ganglia (CG) using whole
cell patch-clamp recording. Neuronal precursors devoid of neuron-specific
surface markers were isolated during the period of neuronal birth, i.e., at
embryonic day (E) 6 from DRG and at E4.5 from CG. All neuronal precursor
cells from DRG, as well as CG, showed outward K+ currents directly after
they had attached to the substrate. During the first 5 hr in culture, half
of the DRG cells had no inward currents at all, whereas the other half
displayed a rapidly and fully inactivating Ca2+ current, which was
activated with small depolarizing pulses from a holding potential of -80 mV
to a -50 mV membrane potential (low-voltage-activated current, LVA). At
these early stages, no other inward currents were resolved. TTX-blockable
Na+ currents and slowly inactivating classical Ca2+ currents, which were
activated with larger depolarizing pulses to a -20 mV membrane potential
(high-voltage- activated currents, HVA) appeared concurrently after 15-20
hr in culture. In contrast, more than half of the CG cells showed LVA
currents, as well as Na+ currents, as early as during the first 5 hr in
culture. The HVA Ca2+ currents from the majority of the cells could be
recorded only after 10-15 hr in culture. In both types of precursor-
derived neurons, the LVA Ca2+ current preceded the classical HVA Ca2+
current. However, the temporal relation of the first Na+ currents to the
first HVA Ca2+ currents seemed to be different in the 2 preparations. In
DRG cells, Na+ and HVA Ca2+ currents appeared at the same time, whereas in
CG cells, the HVA Ca2+ current showed a time lag with respect to the Na+
current. In addition, the relative amplitudes of the currents differed in
the CG and DRG cells. This shows that as early as E4-6, shortly after their
terminal mitosis, neurons from distinct peripheral ganglia in chick vary in
the development of their basic ionic currents.
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