WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience The New Axio Examiner
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sherman, T. G.
Right arrow Articles by Watson, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sherman, T. G.
Right arrow Articles by Watson, S. J.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 8, 3797-3811, Copyright © 1988 by Society for Neuroscience

ARTICLE

Differential expression of vasopressin alleles in the Brattleboro heterozygote

TG Sherman and SJ Watson
Mental Health Research Institute, University of Michigan, Ann Arbor 48109.

A solution hybridization/RNase protection assay for the molar quantitation of vasopressin and oxytocin mRNAs, using synthetic complementary RNA probes, is described. This assay was optimized to permit the identification of vasopressin (AVP) mRNAs containing the frame-shift point deletion causing inheritable diabetes insipidus in the Brattleboro strain of rat. Examination of RNA from hypothalamic magnocellular tissue punches found that of the 86.1 x 10(-18) mol [86.1 attomoles (amol)] of AVP mRNA detected in the Brattleboro heterozygote paraventricular (PVN) nucleus, 5.2% could be shown to be mutant AVP mRNA (AVPd RNA). The percentage of AVPd RNA increased dramatically to 18.1% after 6 d of chronic intermittent salt-loading. Similar percentages and percentage increases of AVPd RNA were detected in the heterozygote supraoptic nucleus (SON). These values were contrasted with those found in parallel studies in both Long Evans and Brattleboro homozygotes, and compared with values for oxytocin (OT) mRNA in all 3 AVP rat genotypes. The results of continued osmotic regulation of the mutant AVP gene, the low native levels of AVPd RNA found in both the Brattleboro heterozygote and homozygote, and the magnitudes of AVPd expression change with chronic osmotic challenge were interpreted as indicating that (1) in the diploid rat genome, both AVP alleles are transcribed, (2) the osmotic regulation of the mutant AVP gene is normal, and (3) the low levels of AVPd mRNA are consistent with a shorter-than-control effective mRNA half-life.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. M. Taylor, J. R. Baker, and W. K. Samson
Brain-derived adrenomedullin controls blood volume through the regulation of arginine vasopressin production and release
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1203 - R1210.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. P. H. Burbach, S. M. Luckman, D. Murphy, and H. Gainer
Gene Regulation in the Magnocellular Hypothalamo-Neurohypophysial System
Physiol Rev, July 1, 2001; 81(3): 1197 - 1267.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. Xi, K. Kusano, and H. Gainer
Quantitative Analysis of Oxytocin and Vasopressin Messenger Ribonucleic Acids in Single Magnocellular Neurons Isolated from Supraoptic Nucleus of Rat Hypothalamus
Endocrinology, October 1, 1999; 140(10): 4677 - 4682.
[Abstract] [Full Text]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-