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Journal of Neuroscience, Vol 8, 3812-3821, Copyright © 1988 by Society for Neuroscience
Electrophysiology of morphologically identified mossy cells of the dentate hilus recorded in guinea pig hippocampal slices
HE Scharfman and PA Schwartzkroin
Department of Neurological Surgery, University of Washington, Seattle 98195.
A specific population of cells located in the hilus of the hippocampal
fascia dentata was studied in guinea pig hippocampal slices using standard
intracellular recording techniques. Twenty-one such cells were
characterized using electrophysiological techniques and were identified
morphologically as mossy cells following intracellular injection of the
fluorescent dye Lucifer yellow. These cells had a resting membrane
potential (mean, -64.6 mV), action potential amplitude (mean, 78.6 mV),
action potential duration (mean, 2.2 msec), and time constant (mean, 24.2
msec) similar to those of hippocampal pyramidal cells of area CA3.
Rectification seen in their I-V curves, and their ability to fire action
potentials in accommodating trains or bursts in response to injected
current pulses, were also similar to those of area CA3 pyramidal cells.
However, these cells could be distinguished from area CA3 pyramidal cells
by their higher input resistance (mean, 97.4 M omega) and higher level of
spontaneous activity. The synaptic responses of mossy cells were also
different from those of CA3 pyramidal cells. First, mossy cells responded
to low levels of stimulation in all areas of the hippocampal slice that
were tested, even areas as remote as area CA1. Second, the responses of
mossy cells to stimulation consisted primarily of EPSPs. Hyperpolarizing
IPSP-like events followed EPSPs in some cells, but the hyperpolarizations
were small and monophasic, even after the cell was depolarized with current
injection. This response contrasts with the smaller EPSP and the prominent,
biphasic IPSP elicited by afferent stimulation of area CA3 pyramidal cells.
The physiological and morphological characteristics of these cells suggest
that they could play an important role in the integration of electrical
activity in the hippocampus.
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