 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 8, 4470-4481, Copyright © 1988 by Society for Neuroscience
A slowly inactivating potassium current truncates spike activity in ganglion cells of the tiger salamander retina
P Lukasiewicz and F Werblin
Neurobiology Group, University of California, Berkeley 94720.
Voltage-gated ganglion cell membrane currents were studied under whole-
cell patch clamp in isolation and in retinal slices. The cells were
identified by (1) backfilling their axons with rhodamine and later
identifying them by their fluorescence in the slice or the mix of isolated
cells or (2) by filling them with Lucifer yellow during recording in
retinal slices. Both methods yielded cells with similar currents. In some
cases, isolated cells lacked processes yet showed currents similar to other
cells, suggesting that voltage-gated currents in all cells were located
primarily at the soma. Both a conventional inactivating sodium current and
a sustained calcium current were found. We describe 3 inactivating outward
currents, ordered in their rate of inactivation. The fastest current
resembled IA reported by Connor and Stevens (1971a, b). A slower current
labeled IB inactivated with a time constant of 339 msec at 0 mV. The
current with slowest inactivation is labeled IC here, inactivating with a
time constant of 4.03 sec at 0 mV. An additional outward current was
sustained and calcium dependent labeled IK(Ca). IB was the largest of these
currents. It was slower than IA, was not blocked by 4AP, and inactivated
over a much more positive potential range. IB appears to play an important
role in spike generation, different from that of IA: Its inactivation leads
to a slow depolarizing shift of the membrane during a current step,
truncating spike activity after about 300-700 msec as the membrane
potential enters the region of sodium inactivation. We analyze how the
inactivating outward current acts to ensure a graded spiking response and
to truncate the spiking output in the presence of large excitatory inputs.
This article has been cited by other articles:
|
|
|
|
 
M. Kaneda, K. Ito, Y. Morishima, Y. Shigematsu, and Y. Shimoda
Characterization of Voltage-Gated Ionic Channels in Cholinergic Amacrine Cells in the Mouse Retina
J Neurophysiol,
June 1, 2007;
97(6):
4225 - 4234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. C. Lee and A. T. Ishida
Ih Without Kir in Adult Rat Retinal Ganglion Cells
J Neurophysiol,
May 1, 2007;
97(5):
3790 - 3799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ichinose, C. R. Shields, and P. D. Lukasiewicz
Sodium Channels in Transient Retinal Bipolar Cells Enhance Visual Responses in Ganglion Cells
J. Neurosci.,
February 16, 2005;
25(7):
1856 - 1865.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.C.W. van Rossum, B. J. O'Brien, and R. G. Smith
Effects of Noise on the Spike Timing Precision of Retinal Ganglion Cells
J Neurophysiol,
May 1, 2003;
89(5):
2406 - 2419.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. R. Shields and P. D. Lukasiewicz
Spike-Dependent GABA Inputs to Bipolar Cell Axon Terminals Contribute to Lateral Inhibition of Retinal Ganglion Cells
J Neurophysiol,
May 1, 2003;
89(5):
2449 - 2458.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. J. Kim and F. Rieke
Slow Na+ Inactivation and Variance Adaptation in Salamander Retinal Ganglion Cells
J. Neurosci.,
February 15, 2003;
23(4):
1506 - 1516.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Tabata and M. Kano
Heterogeneous Intrinsic Firing Properties of Vertebrate Retinal Ganglion Cells
J Neurophysiol,
January 1, 2002;
87(1):
30 - 41.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. R. Shields, M. N. Tran, R. O. L. Wong, and P. D. Lukasiewicz
Distinct Ionotropic GABA Receptors Mediate Presynaptic and Postsynaptic Inhibition in Retinal Bipolar Cells
J. Neurosci.,
April 1, 2000;
20(7):
2673 - 2682.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hirooka, D. E. Kourennyi, and S. Barnes
Calcium Channel Activation Facilitated by Nitric Oxide in Retinal Ganglion Cells
J Neurophysiol,
January 1, 2000;
83(1):
198 - 206.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Tabata and A. T. Ishida
A Zinc-Dependent Cl- Current in Neuronal Somata
J. Neurosci.,
July 1, 1999;
19(13):
5195 - 5204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. W. Sheasby and J. F. Fohlmeister
Impulse Encoding Across the Dendritic Morphologies of Retinal Ganglion Cells
J Neurophysiol,
April 1, 1999;
81(4):
1685 - 1698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhang, N. Tian, and M. M. Slaughter
Neuronal Discriminator Formed by Metabotropic gamma -Aminobutyric Acid Receptors
J Neurophysiol,
December 1, 1998;
80(6):
3365 - 3368.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D. Lukasiewicz and C. R. Shields
Different Combinations of GABAA and GABAC Receptors Confer Distinct Temporal Properties to Retinal Synaptic Responses
J Neurophysiol,
June 1, 1998;
79(6):
3157 - 3167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Berry II and M. Meister
Refractoriness and Neural Precision
J. Neurosci.,
March 15, 1998;
18(6):
2200 - 2211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. Fohlmeister and R. F. Miller
Impulse Encoding Mechanisms of Ganglion Cells in the Tiger Salamander Retina
J Neurophysiol,
October 1, 1997;
78(4):
1935 - 1947.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. Fohlmeister and R. F. Miller
Mechanisms by Which Cell Geometry Controls Repetitive Impulse Firing in Retinal Ganglion Cells
J Neurophysiol,
October 1, 1997;
78(4):
1948 - 1964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Han, J. Zhang, and M. M. Slaughter
Partition of Transient and Sustained Inhibitory Glycinergic Input to Retinal Ganglion Cells
J. Neurosci.,
May 15, 1997;
17(10):
3392 - 3400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D. Lukasiewicz, J. A. Wilson, and J. E. Lawrence
AMPA-Preferring Receptors Mediate Excitatory Synaptic Inputs to Retinal Ganglion Cells
J Neurophysiol,
January 1, 1997;
77(1):
57 - 64.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
