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Journal of Neuroscience, Vol 8, 4780-4789, Copyright © 1988 by Society for Neuroscience
cDNA cloning and characterization of three genes uniquely expressed in cerebellum by Purkinje neurons
DT Nordquist, CA Kozak and HT Orr
Institute of Human Genetics, University of Minnesota, Minneapolis 55455.
The characteristics that distinguish the different neuronal cell types of
an organism are believed to be primarily determined by unique patterns of
cellular gene expression. The identification of cell-type specific
molecules should therefore provide a good basis for understanding the
biology of specific neuron types. In this paper, we describe the isolation
of cDNA clones corresponding to mRNA uniquely expressed by Purkinje cells
in mature mouse cerebellum. Three cDNA clones were selected from a library
of normal mouse cerebellar cDNA by virtue of their failure to hybridize to
mRNA sequences from the cerebella of Purkinje cell degeneration (pcd) mice.
The cDNA clones were shown by in situ and Northern hybridization to
correspond with mRNA present in Purkinje cells but absent or at low levels
in other cell types of the cerebellum. By sequence analysis, clone PCD29
was determined to encode the calcium-binding protein calbindin-D28K. Clones
PCD5 and PCD6 encode previously undescribed proteins of 99 and greater than
500 amino acids, respectively. All 3 PCD clones hybridized to mouse mRNA
from sources other than cerebellum; clone PCD5 was found to have the most
restricted expression, as it hybridized only to mRNA from cerebellum and
eye. To define potential correlations between the PCD clones and mutations
in the mouse genome known to affect Purkinje cells, clones PCD5, PCD6, and
PCD29 were localized to mouse chromosomes 8, 6, and 4, respectively.
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