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Journal of Neuroscience, Vol 8, 555-563, Copyright © 1988 by Society for Neuroscience
Distribution of a novel 57 kDa intermediate filament (IF) protein in the nervous system
LM Parysek and RD Goldman
Northwestern University Medical School, Department of Cell Biology and Anatomy, Chicago, Illinois 60611.
A 57 kDa protein, that is not vimentin, is the major component of
intermediate filaments (IF) obtained after 2 cycles of in vitro assembly
from PC12 cells (Parysek and Goldman, 1987). By use of an antiserum to the
57 kDa protein, a cross-reacting antigen (of identical molecular weight)
was detected on immunoblots of IF preparations and by immunofluorescence of
various rat tissues. Immunolocalization studies on 3-4 micron frozen
sections of tongue, small intestine, and adrenal gland showed bright
labeling of nerve bundles and fine-caliber nerve fibers. The chromaffin
cells and ganglion cells of the adrenal medulla also were labeled. In the
nervous system, intense labeling was seen in small-caliber nerve fibers in
sciatic nerve and spinal cord dorsal roots, as well as in the dorsal white
columns and dorsal root ganglia. Of the ganglion cells, preferential
labeling was seen in small-sized ganglion cells, whereas a monoclonal
antibody to the 150 and 200 kDa neurofilament triplet (NFT) components
labeled the large-sized ganglion cells. In the areas of the brain thus far
examined with 57 kDa antiserum, there was labeling of components of cranial
nerves and labeling of thin fibers in several areas, including the granular
layer of the cerebellum and the corticospinal tract in the brain stem. For
each tissue, adjacent sections treated with vimentin or glial fibrillary
acidic protein antibody revealed labeling patterns distinct from that seen
with either the 57 kDa or NFT antibodies. These results indicate that the
57 kDa IF protein is a neural IF component. Furthermore, this protein is
distributed in only certain neuronal elements; these elements may be
unified by an as yet unrecognized pattern of function in the nervous
system.
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