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Journal of Neuroscience, Vol 8, 664-681, Copyright © 1988 by Society for Neuroscience
Expression of nerve growth factor receptors by Schwann cells of axotomized peripheral nerves: ultrastructural location, suppression by axonal contact, and binding properties
M Taniuchi, HB Clark, JB Schweitzer and EM Johnson Jr
Department of Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110.
Axotomy of sciatic nerve fibers in adult rats induces expression of NGF
receptor in the entire population of Schwann cells located distal to the
injury (Taniuchi et al., 1986b). In the present study we have used
immunocytochemistry, with a monoclonal antibody directed against the rat
NGF receptor, to examine axotomized peripheral nerves by light and electron
microscopy. We have found that (1) the NGF receptor molecules were
localized to the cell surface of Schwann cells forming bands of Bungner;
(2) axonal regeneration into the distal portion of sciatic nerve coincided
temporally and spatially with a decrease in Schwann cell expression of NGF
receptor; (3) Schwann cell NGF receptor could be induced by axotomy of
NGF-independent neurons, such as motoneurons and parasympathetic neurons;
and (4) the presence of axon-Schwann cell contact was inversely related to
expression of Schwann cell NGF receptor. Using biochemical assays we have
found that, in striking contrast to peripheral nerves, there was no
detectable induction of NGF receptor in the spinal cord and brain after
axotomy of NGF receptor- bearing fibers. Filtration assays of 125I-NGF
binding to the induced NGF receptors of Schwann cells measured a Kd of 1.5
nM and a fast dissociation rate, both characteristics of class II receptor
sites. We conclude that Wallerian degeneration induces Schwann cells, but
not central neuroglia, to produce and position upon their plasmalemmal
surface the class II NGF receptor molecules. The induction is ubiquitous
among Schwann cells, irrespective of the type of axon they originally
ensheathed. Expression of Schwann cell NGF receptor is negatively regulated
by axonal contact, being induced when axons degenerate and suppressed when
regenerating axons grow out along the Schwann cell surface. We propose that
the induced NGF receptors function to bind NGF molecules upon the Schwann
cell surface and thereby provide a substratum laden with trophic support
and chemotactic guidance for regenerating sensory and sympathetic neurons.
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