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Journal of Neuroscience, Vol 8, 1063-1073, Copyright © 1988 by Society for Neuroscience
Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture
MW McBurney, KR Reuhl, AI Ally, S Nasipuri, JC Bell and J Craig
Department of Medicine, University of Ottawa, Canada.
We have previously shown that retinoic acid-treated cultures of the P19
line of embryonal carcinoma cells differentiate into neurons, glia, and
fibroblast-like cells (Jones-Villeneuve et al., 1982). We report here that
the monoclonal antibody HNK-1 reacts with the neurons at a very early stage
of their differentiation and is, therefore, an early marker of the neuronal
lineage. Cells in differentiated P 19 cultures synthesized acetylcholine
but not catecholamines, suggesting that at least some of the neurons are
cholinergic. The neurons also carry high- affinity uptake sites for GABA
but not for serotonin. In long-term cultures, neuronal processes
differentiated into axons and dendrites, which formed synapses. This
biological system should prove valuable for examining the development and
maturation of cholinergic neurons, since their differentiation occurs in
cell culture.
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