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Journal of Neuroscience, Vol 8, 1400-1410, Copyright © 1988 by Society for Neuroscience
Stratum lacunosum-moleculare interneurons of hippocampal CA1 region. I. Intracellular response characteristics, synaptic responses, and morphology
JC Lacaille and PA Schwartzkroin
Department of Neurological Surgery, University of Washington, Seattle 98195.
Stable intracellular recordings were obtained from nonpyramidal cells
(interneurons) in stratum lacunosum-moleculare (L-M) of the CA1 region of
guinea pig hippocampal slices. The intracellular response characteristics
of these interneurons were distinctly different from responses of pyramidal
cells and of other interneurons (basket cells and oriens-alveus
interneurons). L-M interneurons had a high resting membrane potential (-58
mV), a high input resistance (64 M omega), and a large amplitude (60 mV),
relatively long duration (2 msec) action potential. A large
afterhyperpolarization (11 mV, 34 msec) followed a single action potential.
Most L-M interneurons did not display any spontaneous firing. Lucifer
yellow (LY)-filled L-M interneurons showed nonpyramidal morphology. Cells
were generally fusiform or multipolar, with aspinous, beaded dendritic
processes ramifying in stratum lacunosum-moleculare, radiatum, and
(sometimes) oriens. The varicose axon originated from a primary dendrite,
projected along stratum lacunosum-moleculare, branched profusely in stratum
radiatum, and coursed toward and into stratum pyramidale and occasionally
into oriens. Processes of cells with somata in the L-M region of CA1 were
not restricted to the CA1 region. The dendritic and axonal processes of
some L-M interneurons were seen ascending in stratum lacunosum- moleculare,
crossing the hippocampal fissure, and coursing in stratum moleculare of the
dentate gyrus. Excitatory and inhibitory postsynaptic potentials (EPSPs and
IPSPs) were evoked in L-M interneurons from stimulation of major
hippocampal afferents. EPSPs were most effectively elicited by stimulation
of fiber pathways in transverse slices, whereas IPSPs were predominantly
evoked when major pathways were stimulated in longitudinal slices. We have
identified a population of interneurons with intracellular response
characteristics and morphology distinctly different from previously
described pyramidal and nonpyramidal neurons of CA1 region. The possible
role of these interneurons in hippocampal circuitry is discussed.
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