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Journal of Neuroscience, Vol 8, 1759-1766, Copyright © 1988 by Society for Neuroscience

ARTICLE

Light- and electron-microscopical study of phosphoprotein B-50 following denervation and reinnervation of the rat soleus muscle

J Verhaagen, AB Oesteicher, PM Edwards, H Veldman, FG Jennekens and WH Gispen
Department of Neurology, University Hospital, Utrecht, The Netherlands.

The neuron-specific phosphoprotein B-50 was originally identified as a phosphoprotein in synaptic plasma membranes isolated from adult brain tissue. In this paper we study the reinnervation of the soleus muscle, a target muscle of sciatic nerve axons, using affinity-purified anti-B- 50 antibodies. Light-microscopical evaluation of the reinnervation process revealed that the period of muscle fiber reinnervation corresponds closely with the time in which high B-50 immunoreactivity was observed in the nerve fibers that invade the muscle and in the newly formed neuromuscular junctions. Upon completion of reinnervation, B-50 immunoreactivity decreased. In the newly innervating terminals, B- 50 was associated with presynaptic vesicular structures and with the presynaptic plasma membrane. In intact mature neuromuscular junctions, virtually no B-50 immunoreactivity could be detected with either light- or electron-microscopic procedures. These observations corroborate the association of high levels of B-50/GAP43 during axon outgrowth and support the concept that B-50 may be a key molecule in the reconstruction of axonal structures. We also observed an unexpected transient increase in B-50 immunoreactivity in the degenerating neuromuscular junctions. This observation cannot be explained in terms of increased neuronal synthesis of B-50, since the degenerating axon processes have been completely disconnected from their cell bodies. Thus, our evidence implies that a rise of B-50 immunoreactivity can be associated with stages of neuronal degeneration as well as with those of neuronal differentiation and axon outgrowth.

This article has been cited by other articles:

				A. J. G. D. Holtmaat, W. T. J. M. C. Hermens, M. A. F. Sonnemans, R. J. Giger, F. W. Van Leeuwen, M. G. Kaplitt, A. B. Oestreicher, W. H. Gispen, and J. Verhaagen Adenoviral Vector-Mediated Expression of B-50/GAP-43 Induces Alterations in the Membrane Organization of Olfactory Axon Terminals In Vivo J. Neurosci., September 1, 1997; 17(17): 6575 - 6586. [Abstract] [Full Text] [PDF]

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