 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 8, 1981-1990, Copyright © 1988 by Society for Neuroscience
Transient expression of laminin in the optic nerve of the developing rat
SC McLoon, LK McLoon, SL Palm and LT Furcht
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis 55455.
The optic nerve of the developing rat was examined for the presence of
laminin, an adhesive glycoprotein, to assess whether it might serve as a
substrate for retinal axon growth in vivo. The optic stalk and nerve of
developing rats were screened immunohistochemically for the presence of
laminin before, during, and after the period of retinal axon growth. On
embryonic day 14 (E14), laminin immunoreactivity was present in the ventral
portion of the optic stalk, the same region in which the first retinal
axons grow. Between E16 and postnatal day 10 (P10), cells positive for
laminin were distributed throughout the cross-sectional area of the nerve.
There was a progressive appearance of glial fibrillary acidic protein
(GFAP) immunoreactivity, a marker for astrocytes, from the optic nerve head
towards the chiasm beginning on E20. At the advancing front of GFAP
immunoreactivity, cells were positive for both laminin and GFAP. Behind the
front, laminin immunoreactivity disappeared from the cells. By P12, the
only laminin immunoreactivity that remained within the optic nerve
surrounded the vasculature. This is a time after the last retinal axons
grow through the optic nerve. Monolayer cell cultures were prepared from
perinatal rat optic nerves and processed for immunohistochemistry to
determine which astrocyte type was laminin-positive. Type 1 astrocytes,
which primarily compose the immature nerve, are GFAP-positive,
A2B5-negative, and laminin-positive. Type 2 astrocytes, a major component
of the mature optic nerve, were GFAP-positive, A2B5-positive and laminin-
negative. An extract of developing optic nerve was analyzed by immunoblot
along with laminin purified from Engelbreth-Holm-Swarm (EHS) sarcoma.
Purified laminin ran with SDS-PAGE under reducing conditions as 2 bands
with Mrs of 200,000 and 4000,000. Both bands reacted with antibodies to
laminin. A low-salt extraction of whole optic nerve from E18 rats resulted
in 2 bands with the same Mr as seen with laminin from EHS sarcoma. When
only the inside of the optic nerve (which lacked the basal lamina and
meninges that surround the outside) was processed, there was a dark 200,000
D band, but the 400,000 D band was virtually absent. These results are
consistent with the hypothesis that laminin, or a variant form of laminin,
serves as a substrate for retinal axon growth in the developing rat optic
nerve.
This article has been cited by other articles:
|
|
|
|
 
D.-Y. Wu, J.-Q. Zheng, M. A. McDonald, B. Chang, and J. L. Twiss
PKC Isozymes in the Enhanced Regrowth of Retinal Neurites after Optic Nerve Injury
Invest. Ophthalmol. Vis. Sci.,
June 1, 2003;
44(6):
2783 - 2790.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Freire, F. C. A. Gomes, R. Linden, V. M. Neto, and T. Coelho-Sampaio
Structure of laminin substrate modulates cellular signaling for neuritogenesis
J. Cell Sci.,
March 14, 2003;
115(24):
4867 - 4876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Grimpe, S. Dong, C. Doller, K. Temple, A. T. Malouf, and J. Silver
The Critical Role of Basement Membrane-Independent Laminin gamma 1 Chain during Axon Regeneration in the CNS
J. Neurosci.,
April 15, 2002;
22(8):
3144 - 3160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Sauve, H. Sawai, and M. Rasminsky
Topological Specificity in Reinnervation of the Superior Colliculus by Regenerated Retinal Ganglion Cell Axons in Adult Hamsters
J. Neurosci.,
February 1, 2001;
21(3):
951 - 960.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Blaschuk, E. Frost, and C ffrench-Constant
The regulation of proliferation and differentiation in oligodendrocyte progenitor cells by alphaV integrins
Development,
January 5, 2000;
127(9):
1961 - 1969.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R. Bansal, S. Winkler, and S. Bheddah
Negative Regulation of Oligodendrocyte Differentiation by Galactosphingolipids
J. Neurosci.,
September 15, 1999;
19(18):
7913 - 7924.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. Farwell and S. A. Dubord-Tomasetti
Thyroid Hormone Regulates the Expression of Laminin in the Developing Rat Cerebellum
Endocrinology,
September 1, 1999;
140(9):
4221 - 4227.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. L. Malosio, D. Gilardelli, S. Paris, C. Albertinazzi, and I. de Curtis
Differential Expression of Distinct Members of Rho Family GTP-Binding Proteins during Neuronal Development: Identification of Rac1B, a New Neural-Specific Member of the Family
J. Neurosci.,
September 1, 1997;
17(17):
6717 - 6728.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L Garcia-Alonso, R. Fetter, and C. Goodman
Genetic analysis of Laminin A in Drosophila: extracellular matrix containing laminin A is required for ocellar axon pathfinding
Development,
January 9, 1996;
122(9):
2611 - 2621.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R Milner and C Ffrench-Constant
A developmental analysis of oligodendroglial integrins in primary cells: changes in alpha v-associated beta subunits during differentiation
Development,
January 12, 1994;
120(12):
3497 - 3506.
[Abstract]
[PDF]
|
|
|
|
