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Journal of Neuroscience, Vol 8, 2618-2627, Copyright © 1988 by Society for Neuroscience
Basic and acidic fibroblast growth factors have trophic effects on neurons from multiple CNS regions
PA Walicke
Developmental Neurobiology Laboratory, Salk Institute for Biological Studies, San Diego, California.
Basic fibroblast growth factor (bFGF) supports the survival of neurons from
many regions of the E18 fetal rat brain. Survival was significantly
increased for neurons derived from the hippocampus, entorhinal cortex (EC),
frontal cortex, parietal cortex (PC), occipital cortex, striatum, septum,
and thalamus, but not from the subiculum (Sb). The proportion of neurons
rescued by bFGF varied among brain regions, suggesting the existence of
subpopulations of responsive neurons. Like hippocampal neurons, neurons
from the EC and PC required about 1 pM bFGF (10-20 pg/ml) for half-maximal
response; striatal neurons, in contrast, required about 3 pM bFGF. Neurite
outgrowth after 24 hr exposure was significantly increased for neurons from
the hippocampus, EC, and PC, while striatal neurons had only a marginal
response. Although bFGF stimulated some astrocytic proliferation in the
cultures, glial contamination was maintained at 2% or less. Acidic FGF
(aFGF) supported smaller numbers of neurons from each region, although it
significantly increased survival of neurons from hippocampus, EC, PC,
striatum, and Sb. The concentration required for half-maximal survival was
around 100-300 pM (2-5 ng/ml). It appears that bFGF and aFGF are potent
trophic factors for many populations of CNS neurons and could potentially
play a significant role in nervous system development.
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