 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 8, 2844-2858, Copyright © 1988 by Society for Neuroscience
Heparan sulfate proteoglycan and laminin immunoreactivity on cultured astrocytes: relationship to differentiation and neurite growth
MD Ard and RP Bunge
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Extracellular matrix (ECM) produced by Schwann cells is known to promote
growth of several types of neurites (Ard et al., 1987). Whether a similar
material produced by astrocytes may be available to promote neurite growth
during CNS development is now open to question. The present study was
undertaken to define conditions under which cultured astrocytes deposit the
ECM components laminin and heparan sulfate proteoglycan (HSPG), and to
relate this deposition to the ability of astrocytes to support neurite
growth. The use of 2 different culture media permitted the growth of
astrocytes either with or without these ECM components. Neonatal rat
cortical astrocytes were cultured by the method of McCarthy and de Vellis
(1980) and studied by immunocytochemistry and electron microscopy.
Astrocytes grown in serum- containing medium for 5 or 9 d after
subculturing were shown to have fibrillar patches of ECM containing both
HSPG and laminin immunoreactivity. Immunoreactivity for the 2 molecules was
usually colocalized. In contrast, astrocytes subcultured for 5 d in defined
medium showed no immunocytochemical staining for either laminin or HSPG and
had no ECM visible in EM. Formation of stellate processes was increased
when cells were grown in defined medium compared with that seen in
serum-containing medium, and growth of the population was slower. In 3
other conditions, attainment of stellate morphological differentiation by
the astrocytes was correlated with diminution in immunostaining for ECM
components. (1) In older cultures (30-42 d after subculturing), stellate,
mitotically quiescent cells showed relatively little HSPG or laminin
immunoreactivity. (2) Cultures initially maintained in serum-containing
medium and then converted to defined medium lost much of their
immunoreactivity for ECM components and developed longer processes. (3)
When neurites from fetal rat dorsal root ganglion explants grew across
monolayers of astrocytes in serum- containing medium, diminution of ECM
immunostaining and development of stellate processes were seen in areas
directly contacted by the neurites. ECM-containing laminin and HSPG did not
appear to be necessary for neurites to interact with astrocytes. In defined
medium, in which no ECM was detected, dorsal root ganglion neurites were
found in contact with astrocyte surfaces rather than on the rat tail
collagen substratum on the culture dish.(ABSTRACT TRUNCATED AT 400 WORDS)
This article has been cited by other articles:
|
|
|
|
 
Y. Eshed, K. Feinberg, D. J. Carey, and E. Peles
Secreted gliomedin is a perinodal matrix component of peripheral nerves
J. Cell Biol.,
May 7, 2007;
177(3):
551 - 562.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Freire, F. C. A. Gomes, T. Jotha-Mattos, V. M. Neto, F. C. S. Filho, and T. Coelho-Sampaio
Sialic acid residues on astrocytes regulate neuritogenesis by controlling the assembly of laminin matrices
J. Cell Sci.,
August 15, 2004;
117(18):
4067 - 4076.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Rakic
Developmental and Evolutionary Adaptations of Cortical Radial Glia
Cereb Cortex,
June 1, 2003;
13(6):
541 - 549.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Milner and I. L. Campbell
Cytokines Regulate Microglial Adhesion to Laminin and Astrocyte Extracellular Matrix via Protein Kinase C-Dependent Activation of the alpha 6beta 1 Integrin
J. Neurosci.,
March 1, 2002;
22(5):
1562 - 1572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Lefrancois, C. Fages, M. Peschanski, and M. Tardy
Neuritic Outgrowth Associated with Astroglial Phenotypic Changes Induced by Antisense Glial Fibrillary Acidic Protein (GFAP) mRNA in Injured Neuron-Astrocyte Cocultures
J. Neurosci.,
June 1, 1997;
17(11):
4121 - 4128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Smith-Thomas, J Stevens, J Fok-Seang, A Faissner, J. Rogers, and J. Fawcett
Increased axon regeneration in astrocytes grown in the presence of proteoglycan synthesis inhibitors
J. Cell Sci.,
January 3, 1995;
108(3):
1307 - 1315.
[Abstract]
[PDF]
|
|
|
|
|
|
|
I Fernaud-Espinosa, M Nieto-Sampedro, and P Bovolenta
Differential effects of glycosaminoglycans on neurite outgrowth from hippocampal and thalamic neurones
J. Cell Sci.,
January 6, 1994;
107(6):
1437 - 1448.
[Abstract]
[PDF]
|
|
|
|
|
|
|
L. Smith-Thomas, J Fok-Seang, J Stevens, J. Du, E Muir, A Faissner, H. Geller, J. Rogers, and J. Fawcett
An inhibitor of neurite outgrowth produced by astrocytes
J. Cell Sci.,
January 6, 1994;
107(6):
1687 - 1695.
[Abstract]
[PDF]
|
|
|
|
.gif?ad=17923&adview=true)
