Journal of Neuroscience, Vol 8, 2961-2966, Copyright © 1988 by Society for Neuroscience
The adhesion molecule on glia (AMOG) incorporated into lipid vesicles binds to subpopulations of neurons
H Antonicek and M Schachner
Department of Neurobiology, University of Heidelberg, FRG.
To investigate the functional role of the novel adhesion molecule on glia
(AMOG) in cell surface interactions, immunoaffinity-purified AMOG was
incorporated into liposomes and measured for its ability to bind to cells
in monolayer cultures. AMOG could be incorporated into liposomes in
functionally active form after solubilization from membranes in 1% cholate
buffer containing soybean lecithin, elution from the AMOG monoclonal
antibody column with 4 M MgCl2, containing 1% octylglucoside, and removal
of detergent for liposome incorporation by gel filtration. AMOG-containing
liposomes bound to neurons, but not to oligodendrocytes, astrocytes, or
fibroblasts in early postnatal cerebellar cultures. AMOG-containing
liposomes also bound to the pheochromocytoma cell line PC12, but not to
neurons in cultures of spinal cord and dorsal root ganglia after various
times in vitro. Fab fragments of monoclonal AMOG antibodies, but not of L3
monoclonal antibodies directed against a carbohydrate structure on AMOG,
inhibited binding of liposomes. Liposome binding was not reduced by
preincubation of cerebellar cells with antibodies to AMOG, to the neuron
adhesion molecule L1, the neural cell adhesion molecule N-CAM, or the L3
carbohydrate structure, nor with 2 monoclonal antibodies reacting with
neuronal cell surface glycoproteins related to the L2/HNK-1 family. These
results show that AMOG is indeed a ligand in adhesion and binds to
particular subpopulations of neurons in L1- and N-CAM-independent
mechanisms.
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