WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (124)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wagner, J. A.
Right arrow Articles by Snyder, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wagner, J. A.
Right arrow Articles by Snyder, S. H.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*CALCIUM COMPOUNDS
*CALCIUM, ELEMENTAL

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 8, 3354-3359, Copyright © 1988 by Society for Neuroscience

ARTICLE

Omega-conotoxin GVIA binding to a high-affinity receptor in brain: characterization, calcium sensitivity, and solubilization

JA Wagner, AM Snowman, A Biswas, BM Olivera and SH Snyder
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

We describe unique, high-affinity binding sites for omega[125I]conotoxin GVIA in membranes from rat brain and rabbit sympathetic ganglia which appear to be primarily associated with N-type voltage-dependent calcium channels. The dissociation constant (KD) for the toxin in rat brain membranes is 60 pM. Physiologic extracellular concentrations of calcium inhibit toxin binding noncompetitively (IC50 = 0.2 mM). The regional distribution of the binding sites in rat brain differs markedly from that of dihydropyridine calcium antagonist receptors associated with L-type calcium channels. In detergent- solubilized brain membranes, toxin binding retains the same affinity, specificity, and ionic sensitivity as in particulate preparations.


This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
L. R. P. Troncone, J. Georgiou, S.-Y. Hua, D. Elrick, I. Lebrun, F. Magnoli, and M. P. Charlton
Promiscuous and Reversible Blocker of Presynaptic Calcium Channels in Frog and Crayfish Neuromuscular Junctions From Phoneutria nigriventer Spider Venom
J Neurophysiol, November 1, 2003; 90(5): 3529 - 3537.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Liang and K. S. Elmslie
Rapid and Reversible Block of N-Type Calcium Channels (CaV 2.2) by omega -Conotoxin GVIA in the Absence of Divalent Cations
J. Neurosci., October 15, 2002; 22(20): 8884 - 8890.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. Shah and D. G. Haylett
Ca2+ Channels Involved in the Generation of the Slow Afterhyperpolarization in Cultured Rat Hippocampal Pyramidal Neurons
J Neurophysiol, May 1, 2000; 83(5): 2554 - 2561.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
T. McMahon, R. Andersen, P. Metten, J. C. Crabbe, and R. O. Messing
Protein Kinase C epsilon Mediates Up-Regulation of N-Type Calcium Channels by Ethanol
Mol. Pharmacol., January 1, 2000; 57(1): 53 - 58.
[Abstract] [Full Text]

Home page
 CirculationHome page
R. J. Gumina, E. Buerger, C. Eickmeier, J. Moore, J. Daemmgen, and G. J. Gross
Inhibition of the Na+/H+ Exchanger Confers Greater Cardioprotection Against 90 Minutes of Myocardial Ischemia Than Ischemic Preconditioning in Dogs
Circulation, December 21, 1999; 100(25): 2519 - 2526.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Lu and K. Dunlap
Cloning and Functional Expression of Novel N-type Ca2+ Channel Variants
J. Biol. Chem., December 3, 1999; 274(49): 34566 - 34575.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y.-F. Xu, S. J. Hewett, and W. D. Atchison
Passive Transfer of Lambert-Eaton Myasthenic Syndrome Induces Dihydropyridine Sensitivity of ICa in Mouse Motor Nerve Terminals
J Neurophysiol, September 1, 1998; 80(3): 1056 - 1069.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. W. McEnery, T. D. Copeland, and C. L. Vance
Altered Expression and Assembly of N-type Calcium Channel alpha 1B and beta  Subunits in Epileptic lethargic (lh/lh) Mouse
J. Biol. Chem., August 21, 1998; 273(34): 21435 - 21438.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. L. Vance, C. M. Begg, W.-L. Lee, H. Haase, T. D. Copeland, and M. W. McEnery
Differential Expression and Association of Calcium Channel alpha 1B and beta  Subunits during Rat Brain Ontogeny
J. Biol. Chem., June 5, 1998; 273(23): 14495 - 14502.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. Solem, T. McMahon, and R. O. Messing
Protein Kinase A Regulates Inhibition of N- and P/Q-type Calcium Channels by Ethanol in PC12 Cells
J. Pharmacol. Exp. Ther., September 1, 1997; 282(3): 1487 - 1495.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
O. T. Jones, G. M. Bernstein, E. J. Jones, D. G. M. Jugloff, M. Law, W. Wong, and L. R. Mills
N-Type Calcium Channels in the Developing Rat Hippocampus: Subunit, Complex, and Regional Expression
J. Neurosci., August 15, 1997; 17(16): 6152 - 6164.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Lew, J. P. Flinn, P. K. Pallaghy, R. Murphy, S. L. Whorlow, C. E. Wright, R. S. Norton, and J. A. Angus
Structure-Function Relationships of omega -Conotoxin GVIA. SYNTHESIS, STRUCTURE, CALCIUM CHANNEL BINDING, AND FUNCTIONAL ASSAY OF ALANINE-SUBSTITUTED ANALOGUES
J. Biol. Chem., May 2, 1997; 272(18): 12014 - 12023.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. Mahata, S. K. Mahata, R. J. Parmer, and D. T. O'Connor
Vesicular Monoamine Transport Inhibitors: Novel Action at Calcium Channels to Prevent Catecholamine Secretion
Hypertension, September 1, 1996; 28(3): 414 - 420.
[Abstract] [Full Text]

Home page
 ScienceHome page
D. Witcher, M De Waard, J Sakamoto, C Franzini-Armstrong, M Pragnell, S. Kahl, and K. Campbell
Subunit identification and reconstitution of the N-type Ca2+ channel complex purified from brain
Science, July 23, 1993; 261(5120): 486 - 489.
[Abstract] [PDF]

Home page
 ScienceHome page
H Komuro and P Rakic
Selective role of N-type calcium channels in neuronal migration
Science, August 7, 1992; 257(5071): 806 - 809.
[Abstract] [PDF]

Home page
 ScienceHome page
M. Williams, P. Brust, D. Feldman, S Patthi, S Simerson, A Maroufi, A. McCue, G Velicelebi, S. Ellis, and M. Harpold
Structure and functional expression of an omega-conotoxin-sensitive human N-type calcium channel
Science, July 17, 1992; 257(5068): 389 - 395.
[Abstract] [PDF]

Home page
 J PsychopharmacolHome page
P. H. Silverstone and D. G. Grahame-Smith
Smithkline Beecham Prize for Young Psychopharmacologists: A review of the relationship between calcium channels and psychiatric disorders
J Psychopharmacol, January 1, 1992; 6(4): 462 - 482.
[Abstract] [PDF]

Home page
 ScienceHome page
B. Olivera, J Rivier, C Clark, C. Ramilo, G. Corpuz, F. Abogadie, E. Mena, Woodward SR, D. Hillyard, and L. Cruz
Diversity of Conus neuropeptides
Science, July 20, 1990; 249(4966): 257 - 263.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
L. Yan and M. E. Adams
The Spider Toxin omega -Aga IIIA Defines a High Affinity Site on Neuronal High Voltage-activated Calcium Channels
J. Biol. Chem., July 7, 2000; 275(28): 21309 - 21316.
[Abstract] [Full Text] [PDF]


-
-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-