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Journal of Neuroscience, Vol 9, 258-279, Copyright © 1989 by Society for Neuroscience
Autoradiographic localization and characterization of tachykinin receptor binding sites in the rat brain and peripheral tissues
PW Mantyh, T Gates, CR Mantyh and JE Maggio
Center for Ulcer Research and Education, VA Medical Center-Wadsworth, Los Angeles, California 90073.
Quantitative receptor autoradiography using several radiolabeled
tachykinins was used to localize and characterize tachykinin peptide
receptor binding sites in rat CNS and peripheral tissues. Autoradiographic
localization and displacement experiments using several radiolabeled
tachykinins indicate that in the rat there are at least 3 distinct
tachykinin receptor binding sites. One of these is present in both the CNS
and peripheral tissues, one is present only in the CNS, and one is present
only in peripheral tissues. The first tachykinin receptor binding site,
which is detectable in both the CNS and peripheral tissues, appears to
prefer substance P (SP) as an endogenous ligand. Areas expressing high
concentrations of this binding site include the medial septum, superior
colliculus, inferior olive, inner plexiform layer of the retina, external
muscle of the bladder, and the muscularis externa of the esophagus. The
second type of tachykinin receptor binding site, which is detectable only
in the CNS appears to prefer either neuromedin K (NK) and/or substance K
(SK) as the endogenous ligand. This receptor binding site is labeled by
Bolton- Hunter conjugates of NK, SK, eledoisin, or kassinin and is found in
high concentrations in laminae 4 and 5 of the cerebral cortex, the ventral
tegmental area, laminae 1 and 2 of the spinal cord, and the inner plexiform
layer of the retina. The third type of tachykinin receptor binding site is
detectable only in peripheral tissues and appears to prefer SK as the
endogenous ligand. This receptor binding site is labeled by SK, eledoisin,
or kassinin radioligands and tissues that express high concentrations
include the muscularis mucosae of the esophagus, the circular muscle of the
colon, and the external muscle of the bladder. These data suggest that SP
receptors are expressed in the brain and peripheral tissues, NK receptors
are expressed in the CNS, and SK receptors are expressed in peripheral
tissue. These data fit well with radioimmunoassay data that suggest that,
whereas in the CNS SP, SK and NK are present in high concentrations, in
peripheral tissues only SP and SK are present in detectable concentrations.
The present classification of tachykinin receptors places a lower limit on
the number of mammalian tachykinin receptor types and provides a
functional/morphological framework for exploring the diverse actions of
tachykinin peptides in both the CNS and peripheral tissues.
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