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Journal of Neuroscience, Vol 9, 3547-3557, Copyright © 1989 by Society for Neuroscience
The adult rat olfactory system expresses microtubule-associated proteins found in the developing brain
C Viereck, RP Tucker and A Matus
Friedrich Miescher-Institut, Basel, Switzerland.
We have compared the expression and localization of neuronal
microtubule-associated proteins (MAPs) MAP5, MAP2, and tau in the adult rat
olfactory system and cerebral cortex. Each of these MAPS is known to exist
as distinct "early" and "late" forms in the developing and adult brain,
respectively. Because axonal growth and dendritic reinnervation continue in
the adult olfactory system, it can serve as a test of whether expression of
early MAP forms is necessary for neuronal growth and plasticity. We found
that for all three MAPs, the early forms continue to be expressed in the
adult olfactory system, whereas the cerebral cortex switches to the late
forms during neuronal maturation (between 10 and 20 days of age in the
rat). For MAP2, the 6 kb mRNA and low-molecular weight MAP2c, both of which
are typically found in embryonic tissue, persist in the adult olfactory
bulb. For MAP5, an early highly phosphorylated form, MAP5a, is present
throughout the brain and disappears during maturation simultaneously with a
several-fold drop in the overall level of MAP5. However, in the olfactory
bulb, MAP5 levels do not fall, and MAP5a persists in the adult. The early
form of tau is also prominent in the adult olfactory bulb and, by
immunohistochemistry, is mainly confined to a subset of olfactory axons,
the vomeronasal nerve. Thus, in the adult olfactory bulb, both MAP protein
synthesis and phosphorylation conform to a pattern associated with the
developing brain. Immunohistochemistry also showed that MAP5 is
concentrated in the olfactory nerve axons and the mitral cell dendrites of
the olfactory bulb, i.e., exactly those elements that are involved in the
olfactory nerve innervation that takes place in the adult. These results
suggest that the expression of the early MAP forms is closely associated
with neurite outgrowth and plasticity.
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