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Journal of Neuroscience, Vol 9, 3638-3644, Copyright © 1989 by Society for Neuroscience

ARTICLE

ARPP-21, a cyclic AMP-regulated phosphoprotein enriched in dopamine- innervated brain regions. II. Molecular cloning and nucleotide sequence

T Kurihara, ME Ehrlich, J Horiuchi, T Nasu and P Greengard
Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021.

A cDNA clone for the mRNA of bovine ARPP-21 (cAMP-regulated phosphoprotein, Mr = 21,000 as determined by SDS-PAGE) was isolated from a modified Okayama-Berg plasmid library. Transformed Escherichia coli colonies were screened by in situ colony hybridization with 2 different oligonucleotide probes derived from the amino acid sequence of the bovine protein. Sequence analysis of the longest cDNA clone, pTKAI [2407 nucleotides plus a poly(A) tail], revealed a 267-nucleotide- long coding region in agreement with the bovine ARPP-21 amino acid sequence (Williams et al., 1989). Southern blot analysis of total bovine genomic DNA raised the possibility that there may be 2 genes coding for ARPP-21. Northern blot analysis of total cellular RNA from bovine caudate nucleus and other brain regions demonstrated the existence of 2 major mRNA species, 2.5 and 1.0 kb in length, probably derived from use of alternate polyadenylation sites. There was a differential expression of these 2 mRNAs within the brain. Both ARPP-21 mRNAs were most abundant in the caudate nucleus, where the concentration of the protein is highly enriched.

This article has been cited by other articles:

				S. Ivkovic and M. E. Ehrlich Expression of the Striatal DARPP-32/ARPP-21 Phenotype in GABAergic Neurons Requires Neurotrophins In Vivo and In Vitro J. Neurosci., July 1, 1999; 19(13): 5409 - 5419. [Abstract] [Full Text] [PDF]

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