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Journal of Neuroscience, Vol 9, 3861-3869, Copyright © 1989 by Society for Neuroscience
B-50/GAP43 is localized at the cytoplasmic side of the plasma membrane in developing and adult rat pyramidal tract
TG Gorgels, M Van Lookeren Campagne, AB Oestreicher, AA Gribnau and WH Gispen
Department of Anatomy and Embryology, University of Nijmegen, The Netherlands.
The neuron-specific phosphoprotein B-50/GAP43 has been implicated in axonal
outgrowth, since high levels of B-50/GAP43 are found in growth cones and
during development of the nervous system. In adult brain, the B-50 levels
are decreased. B-50 is primarily found in axons and presynaptic terminals.
It is phosphorylated by protein kinase C, and this process has been
implicated in the modulation of membrane signal transduction. During the
outgrowth of the pyramidal tract, high levels of B-50 have been reported,
whereas a low amount of B-50 persists into the adult stage. By
immunoelectron microscopy, using immunogold labeling on cryosections and
pre-embedding peroxidase labeling, we examined the distribution of B-50 in
the pyramidal tract at the third cervical segment in developing 2-d-old and
adult 90-d-old rats. B-50 immunoreactivity was found in axons and growth
cones of the outgrowing tract. In the adult pyramidal tract, both
unmyelinated and myelinated axons contained B-50 immunoreactivity. The
immunogold label was predominantly located at the plasma membrane. Since
the peroxidase reaction product was observed exclusively intracellularly,
we conclude that the B-50 immunoreactivity is predominantly located at the
cytoplasmic side of the plasma membrane of axons and growth cones. The high
immunoreactivity in growth cones and axons of the outgrowing pyramidal
tract further supports the hypothesis that B-50 plays a role in neurite
outgrowth. The presence of B-50 in the adult pyramidal tract cannot merely
be attributed to transport to the synapse. Therefore, it is suggested that
B-50 plays, in addition, a local, growth-associated role in the adult
tract.
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