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Journal of Neuroscience, Vol 9, 3988-3997, Copyright © 1989 by Society for Neuroscience
Regulation of tension on hair-cell transduction channels: displacement and calcium dependence
N Hacohen, JA Assad, WJ Smith and DP Corey
Neuroscience Group, Howard Hughes Medical Institute, Boston, Massachusetts.
An epithelial preparation of the bullfrog sacculus was used to characterize
the initial rate of the adaptation mechanism in hair cells and its
dependence on displacement and calcium. The I(X) curve relating
transduction current and bundle displacement shifted along the X-axis
without substantial change in slope, as previously observed, suggesting
that adaptation involves a change in the attachment point of the elastic
element connected to ion channels. If the "tip links" model of transduction
is correct, this implies that one end of the link moves along the side of
the stereocilium. The rates were highly asymmetric: in the tensioning
direction the rate was roughly constant at 1-2 microns/sec (calculated as
motion along a stereocilium); this is similar to that of myosin on actin.
In the relaxing direction it appeared linearly dependent on tension.
Calcium preferentially potentiated the relaxation, and apparently reduced
the resting tension in the elastic element. The calcium site appears
specific for calcium, as other divalent cations inhibited its action.
Dihydrostreptomycin inhibited the positive rate, but its effect could not
be explained by a simple channel block, and it seems inconsistent with
screening of negative charge in the mouth of the transduction channel.
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