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Journal of Neuroscience, Vol 9, 4179-4189, Copyright © 1989 by Society for Neuroscience
Age of differentiation determines rat retinal germinal cell phenotype: induction of differentiation by dissociation
TA Reh and IJ Kljavin
Department of Medical Physiology, Faculty of Medicine, University of Calgary, Alberta, Canada.
We are interested in the mechanisms that control cell phenotype during the
development of the CNS. Since different neuronal types arise at different
times during neurogenesis in the retina, we predicted that the factors that
determine cell type must be developmentally regulated as well. To test this
hypothesis, we induced retinal germinal cells to differentiate at different
ages by dissociating the retina into single cells and culturing them on a
variety of substrates. Prior to dissociation, the S-phase germinal cells
were labeled with 3H-thymidine so that their fate could be specifically
followed. We found that our culture conditions promoted the differentiation
of the majority of the germinal cells and that these cells differentiated
into different neuronal types depending on the age of the animal from which
the retina had been taken; embryonic day 14 germinal cells differentiated
primarily into ganglion cells, and never produced rods, while germinal
cells from postnatal day 1 retina differentiated into rods, but not
ganglion cells. These results are consistent with the hypothesis that
temporally regulated factors determine cell phenotype during the
development of the retina.
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