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Journal of Neuroscience, Vol 9, 4214-4226, Copyright © 1989 by Society for Neuroscience
Localization of potential serotonergic facilitator neurons in Aplysia by glyoxylic acid histofluorescence combined with retrograde fluorescent labeling
RD Hawkins
Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
A variety of evidence suggests that 5-HT participates in presynaptic
facilitation of the siphon sensory cells contributing to dishabituation and
sensitization of the gill- and siphon-withdrawal reflex in Aplysia. Most
recently, Glanzman et al. (1989) have shown that the 5-HT neurotoxin
5,7-DHT markedly reduces both the synaptic facilitation and behavioral
dishabituation produced by tail shock. To provide more direct evidence for
a role of 5-HT, I have used histological techniques to try to locate
individual serotonergic facilitator neurons. I first used a modification of
the glyoxylic acid histofluorescence technique to map serotonergic and
dopaminergic neurons in the CNS of Aplysia. Intracellular fluorescent
labeling combined with histofluorescence indicates that the previously
identified L29 facilitator neurons are not serotonergic. Nerve transection
experiments suggest that most of the perisomatic 5-HT histofluorescence in
the abdominal ganglion (the location of the siphon sensory cells) comes
from neurons whose cell bodies are located in the pedal or cerebral
ganglia. As there are at least 500 serotonergic neurons in those ganglia, I
combined retrograde fluorescent labeling with histofluorescence to identify
a small subset of those neurons which send processes to the abdominal
ganglion and are therefore potential serotonergic facilitators. In the
following paper, Mackey et al. (1989) show that stimulation of 2 of those
neurons in the cerebral ganglia (the CB1 cells) produces presynaptic
facilitation of the siphon sensory cells contributing to dishabituation and
sensitization of the withdrawal reflex.
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