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Journal of Neuroscience, Vol 9, 4227-4235, Copyright © 1989 by Society for Neuroscience
Identified serotonergic neurons LCB1 and RCB1 in the cerebral ganglia of Aplysia produce presynaptic facilitation of siphon sensory neurons
SL Mackey, ER Kandel and RD Hawkins
Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Several lines of evidence suggest that 5-HT plays a significant role in
presynaptic facilitation of the siphon sensory cells contributing to
dishabituation and sensitization of the gill- and siphon-withdrawal reflex
in Aplysia. Most recently, Glanzman et al. (1989) found that treatment with
the 5-HT neurotoxin, 5,7-DHT markedly reduced both synaptic facilitation
and behavioral dishabituation. To provide more direct evidence for a role
of 5-HT, we have attempted to identify individual serotonergic facilitator
neurons. Hawkins (1989) used histological techniques to locate several
serotonergic neurons in the ring ganglia that send axons to the abdominal
ganglion and are therefore possible serotonergic facilitators. These
include one neuron in the B cluster of each cerebral ganglion, which we
have identified electrophysiologically and named the CB1 cells. Both
glyoxylic acid histofluorescence and 5-HT immunofluorescence indicate that
the CB1 neurons are serotonergic. In a semiintact preparation, the CB1
neurons respond to cutaneous stimulation which produces dishabituation and
sensitization (such as tail shock) with an increase in firing, which may
outlast the stimulation by 15 min. Intracellular stimulation of a CB1
neuron in a manner similar to its response to tail shock produces
facilitation of the EPSPs from siphon sensory neurons to motor neurons, as
well as broadening of the action potential in the sensory neurons in
tetraethylammonium solution. These results strongly suggest that the
identified serotonergic CB1 neurons participate in mediating presynaptic
facilitation contributing to dishabituation and sensitization of the gill-
and siphon-withdrawal reflex in Aplysia.
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