QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (186) Citing Articles via Google Scholar Google Scholar Articles by Hoffman, P. N. Search for Related Content PubMed PubMed Citation Articles by Hoffman, P. N.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 9, 893-897, Copyright © 1989 by Society for Neuroscience

ARTICLE

Expression of GAP-43, a rapidly transported growth-associated protein, and class II beta tubulin, a slowly transported cytoskeletal protein, are coordinated in regenerating neurons

PN Hoffman
Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

GAP-43 is a membrane-associated phosphoprotein enriched in elongating axons (Meiri et al., 1986; Skene et al., 1986). After an axon has been interrupted by cutting or crushing a nerve (axotomy), the portion of the axon disconnected from the cell body (distal stump) degenerates and is replaced by the outgrowth (elongation) of regenerating sprouts arising from the proximal stump. Previous studies have shown that increased amounts of pulse-labeled GAP-43 undergo fast axonal transport in regenerating neurons (Benowitz et al., 1981; Skene and Willard, 1981 a, b). Using hybridization with a cloned cDNA probe, I now show that mRNA levels for GAP-43 increase in lumbar sensory neurons of rat after regeneration is initiated by crushing the sciatic nerve; the relatively high levels of GAP-43 mRNA in regenerating neurons are comparable to those in the developing neurons of 5-d-old animals. I further demonstrate that the induction of GAP-43 expression in regenerating neurons coincides temporally with an increase in mRNA levels for class II beta tubulin (Hoffman and Cleveland, 1988), suggesting that the expression of these proteins is closely coordinated during axonal elongation.

This article has been cited by other articles:

				D. Y. Lam, P. L. Kaufman, B'A. T. Gabelt, E. C. To, and J. A. Matsubara Neurochemical Correlates of Cortical Plasticity after Unilateral Elevated Intraocular Pressure in a Primate Model of Glaucoma Invest. Ophthalmol. Vis. Sci., June 1, 2003; 44(6): 2573 - 2581. [Abstract] [Full Text] [PDF]

				V. Prevot, S. Bouret, D. Croix, G. Alonso, L. Jennes, V. Mitchell, A. Routtenberg, and J.-C. Beauvillain Growth-Associated Protein-43 Messenger Ribonucleic Acid Expression in Gonadotropin-Releasing Hormone Neurons during the Rat Estrous Cycle Endocrinology, May 1, 2000; 141(5): 1648 - 1657. [Abstract] [Full Text] [PDF]

				E. C. Johnson, L. M. H. Deppmeier, S. K. F. Wentzien, I. Hsu, and J. C. Morrison Chronology of Optic Nerve Head and Retinal Responses to Elevated Intraocular Pressure Invest. Ophthalmol. Vis. Sci., February 1, 2000; 41(2): 431 - 442. [Abstract] [Full Text]

				R. E. Zigmond {blacksquare} REVIEW : LIF, NGF, and the Cell Body Response to Axotomy Neuroscientist, May 1, 1997; 3(3): 176 - 185. [Abstract] [PDF]

				D. S. Smith and J. H. Pate Skene A Transcription-Dependent Switch Controls Competence of Adult Neurons for Distinct Modes of Axon Growth J. Neurosci., January 15, 1997; 17(2): 646 - 658. [Abstract] [Full Text] [PDF]

				P. N. Hoffman Review : The Synthesis, Axonal Transport, and Phosphorylation of Neurofilaments Determine Axonal Caliber in Myelinated Nerve Fibers Neuroscientist, March 1, 1995; 1(2): 76 - 83. [Abstract] [PDF]

				E Reinhard, E Nedivi, J Wegner, J. Skene, and M Westerfield Neural selective activation and temporal regulation of a mammalian GAP-43 promoter in zebrafish Development, January 7, 1994; 120(7): 1767 - 1775. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help