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Journal of Neuroscience, Vol 9, 990-995, Copyright © 1989 by Society for Neuroscience
Localization of the growth-associated phosphoprotein GAP-43 (B-50, F1) in the human cerebral cortex
LI Benowitz, NI Perrone-Bizzozero, SP Finklestein and ED Bird
Department of Psychiatry, Harvard Medical School, Massachusetts 02178.
The growth-associated phosphoprotein GAP-43 is a component of the
presynaptic membrane that has been linked to the development and functional
modulation of neuronal connections. A monospecific antibody raised against
rat GAP-43 was used here to study the distribution of the protein in
cortical and subcortical areas of the human brain. On Western blots, the
antibody recognized a synaptosomal plasma membrane protein that had an
apparent molecular weight and isoelectric point similar to GAP-43 of other
species. In brain tissue reacted with the antibody, the heaviest
immunoreactivity was found in associative areas of the neocortex,
particularly within layers 1 and 6, in the molecular layer of the dentate
gyrus, the caudate putamen, and the amygdala. In contrast, primary sensory
or motor regions of the cortex, portions of dorsal thalamus, and cerebellum
showed only light staining. Staining was generally confined to the
neuropil, which showed punctate labeling, whereas most neuronal somata and
fiber bundles were unreactive. The pronounced variations in GAP-43
immunostaining among various areas of the human brain may reflect different
potentials for functional and/or structural remodeling.
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