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Journal of Neuroscience, Vol 9, 1937-1942, Copyright © 1989 by Society for Neuroscience
The relationship between the number of synaptic vesicles and the amount of transmitter released
JH Koenig, T Kosaka and K Ikeda
Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California 91010.
The relationship between the number of synaptic vesicles and the amount of
transmitter released from identified synapses was investigated in the
dorsal longitudinal flight muscle (DLM) of the temperature- sensitive
endocytosis mutant of Drosophila melanogaster, shibirets- 1(shi). In the
shi fly at 29 degrees C, vesicle recyling is blocked, but transmitter
release proceeds normally. Thus, by inducing transmitter release at 29
degrees C, shi synapses gradually become depleted of synaptic vesicles. In
this way it was possible to regulate the number of vesicles in a synapse.
Intracellular recordings were made from individual fibers of the DLM in shi
flies after various periods at 29 degrees C while stimulating at 0.5 Hz.
The amplitude of the evoked excitatory junction potential (ejp), gradually
decreased with longer exposure and was brought to various levels. The fiber
was then rapidly fixed for electron microscopy. The number of vesicles per
synapse was compared with the amplitude of the ejp at the time of fixation.
It was observed that the smaller the ejp amplitudes became, the fewer
vesicles were in the synapses. Also, as the ejp amplitude decreased, an
increased number of synapses contained no vesicles. It is concluded that
synaptic vesicles are directly involved in the release process.
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