QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Earnest, D. J. Articles by Wiegand, S. J. Search for Related Content PubMed PubMed Citation Articles by Earnest, D. J. Articles by Wiegand, S. J.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 9, 2671-2677, Copyright © 1989 by Society for Neuroscience

ARTICLE

Specificity of circadian function in transplants of the fetal suprachiasmatic nucleus

DJ Earnest, CD Sladek, DM Gash and SJ Wiegand
Department of Neurobiology and Anatomy, University of Rochester School of Medicine, New York 14642.

Fetal tissues obtained from specific regions of the developing hypothalamus were transplanted to determine whether the precursor neurons of the suprachiasmatic nucleus (SCN) can be distinguished from those of the presumptive paraventricular nucleus (PVN) on the basis of the functional capacity to generate circadian rhythms. The presumptive SCN, the PVN, and a portion of the neocortical primordium were dissected from the developing forebrains of normal Long-Evans fetuses, separated, and selectively transplanted into the periventricular-third ventricle region of adult, vasopressin (VP)-deficient Brattleboro rats. In host animals that received grafts containing the precursor population of SCN neurons, the temporal profile of VP levels in the cerebrospinal fluid (CSF) oscillated with a circadian periodicity in a manner similar to that observed in normal Long-Evans rats. CSF collected serially from animals with grafts of the presumptive PVN also contained VP, but no circadian variation was manifested in peptide levels. VP was undetectable in CSF samples obtained from Brattleboro rats with cortical grafts. In association with their circadian functional capacity, grafts of the SCN primordium were characterized by clusters of parvicellular neurons immunopositive for VP or vasoactive intestinal polypeptide (VIP) that resembled the cell groups of the in situ SCN. In contrast, transplants of the presumptive PVN did not contain neurons immunoreactive for VIP, and the VP neurons in these grafts resembled the neurosecretory cells of the PVN.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				J. Grosse and F. C. Davis Melatonin Entrains the Restored Circadian Activity Rhythms of Syrian Hamsters Bearing Fetal Suprachiasmatic Nucleus Grafts J. Neurosci., October 1, 1998; 18(19): 8032 - 8037. [Abstract] [Full Text] [PDF]

				H. Shen, M. Watanabe, H. Tomasiewicz, U. Rutishauser, T. Magnuson, and J. D. Glass Role of Neural Cell Adhesion Molecule and Polysialic Acid in Mouse Circadian Clock Function J. Neurosci., July 1, 1997; 17(13): 5221 - 5229. [Abstract] [Full Text] [PDF]

				F. C. Davis and N. Viswanathan The Effect of Transplanting One or Two Suprachiasmatic Nuclei on the Period of the Restored Rhythm J Biol Rhythms, December 1, 1996; 11(4): 291 - 301. [Abstract] [PDF]

				J. LeSauter, M. N. Lehman, and R. Silver Restoration of Circadian Rhythmicity by Transplants of SCN "Micropunches" J Biol Rhythms, June 1, 1996; 11(2): 163 - 171. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help