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Journal of Neuroscience, Vol 9, 2866-2877, Copyright © 1989 by Society for Neuroscience
Altered branching of serotonin-containing neurons in Drosophila mutants unable to synthesize serotonin and dopamine
V Budnik, CF Wu and K White
Biophysics Program, Brandeis University, Waltham, Massachusetts 02254.
The anatomy of peripheral serotonin-containing fibers (5-HT fibers) in the
gut of wild-type Drosophila larvae was compared to mutants deficient in the
gene that encodes the enzyme dopa decarboxylase (DfDdc mutants). The 5-HT
fibers, located in the proventriculus and midgut, were visualized
immunocytochemically by using a monoclonal antibody against 5-HT. Since
DfDdc larvae are devoid of 5-HT and dopamine in the nervous system, the
highly selective uptake capability of 5-HT neurons was used to visualize
the 5-HT fibers. We found that the absence of 5- HT and dopamine in the
nervous system of DfDdc animals does not prevent 5-HT fibers from reaching
their appropriate targets. However, these fibers in the mutant show a
2-fold increase in the extent of branching. This effect is specific to 5-HT
fibers, since glutamate-like and FMRFamide-like immunoreactive fibers of
the proventriculus and midgut remain unaffected in the mutant. Low but
detectable levels of dopamine and 5-HT in the CNS are sufficient to prevent
the increase in arborization, as indicated by analyses of a
temperature-sensitive Ddc allele (Ddcts2), which has very low dopa
decarboxylase activity. The abnormally extensive branching of 5-HT fibers
also can be partially rescued by feeding DfDdc larvae with dopamine. In
contrast, feeding with a 5-HT-containing diet had no effect on the mutant
phenotype. Hypotheses that could explain the mutant phenotype are proposed.
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